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Thymic alterations induced by partial hepatectomy: upregulation of glycoprotein 96, CD91 and TLR2 and generation of regulatory T cells

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dc.contributor.authorJakovac, Hrvoje
dc.contributor.authorĆuk, Mira
dc.contributor.authorTrobonjača, Zlatko
dc.contributor.authorMrakovčić-Šutić, Ines
dc.contributor.authorRadošević-Stašić, Biserka
dc.date.accessioned2020-09-21T17:03:43Z
dc.date.available2020-09-21T17:03:43Z
dc.date.issued2015
dc.description.abstractGlycoprotein 96 (gp96) is an endoplasmic reticulum (ER)-resident heat shock protein. It controls the folding of nascent membrane-spanning and secretory proteins, participates in stress-induced unfolded protein response (UPR) and in pathways leading to proteolysis of damaged proteins through ER-associated degradation pathways and chaperone-mediated autophagy. In addition, gp96 controls the steroid biosynthesis and Ca2+ homeostasis and participates in insulin-IGF/signaling pathways. Besides, owing to its peptide chaperone capacity and ability to interact with antigen-presenting cells, gp96 has been implicated in priming of innate and adaptive immunity. In an attempt to visualize the intensity of ER-stress in thymus and possible participation of gp96 in generation of auto-reactive T cell clones that were detected in regenerating liver, in this study we investigated the dynamics of gp96 expression in partially hepatectomized (pHx) and sham Hx mice. Simultaneously, we detected the thymic expression of receptors responsible for endocytosis of gp96- chaperoned peptides (CD91) and intracellular activation of ER-stress pathways (TLR2), as well as the expression of TGF-β and the distribution of CD4+CD25+FoxP3+ cells. The data have shown that both pHx and sham Hx induced an accelerated apoptosis and hypoplasia in thymus. Partial Hx induced, however, a higher expression of gp96, the translocation of the CD91, TLR2 and TGF-β immunostaining from medulla to cortex and an appearance of Treg cells. The data show that pHx triggers in thymus the ERstress and UPR response and suggest that gp96 participates in the generation of natural Treg cells, which might be involved in the control of liver regeneration in the periphery.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.citationHistology and histopathology, Vol. 30, nº 8 (2015)
dc.identifier.doihttps://doi.org/10.14670/HH-11-597
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/96266
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLiver regenerationes
dc.subjectThymuses
dc.subjectGlycoprotein 96es
dc.subjectCD91es
dc.subjectTLR2es
dc.subjectTGF-betaes
dc.subjectRegulatory T cellses
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleThymic alterations induced by partial hepatectomy: upregulation of glycoprotein 96, CD91 and TLR2 and generation of regulatory T cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nº8 (2015)