Publication: Topographical difference of cytoskeletal organization in smooth muscle cells of rat duodenum revealed by quick-freezing and deep-etching method
Loading...
Date
2000
Authors
Takayama, I. ; Fuji, Y. ; Terada, N. ; Baba, T. ; Kato, Y. ; Fujino, M.A. ; Ohno, S.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The sarcolemmal domain of rat duodenal
smooth muscle cells includes caveolae and associated
cytoskeletal or filamentous elements. We have used the
quick-freezing, deep-etching method to examine the
three dimensional relationships between these
components. Replica membranes for separated strips of
rat duodenal muscle layers were routinely prepared after
extraction soluble proteins from cytoplasm and
extracellular matrix. As results, 1) cytoskeletal elements
in smooth muscle cells consisted mainly of striated thin
filaments; 2) thin filaments were connected with some
plasma membranes through filaments associated with the
sarcolemma, which formed fine network structures
beneath the sarcolemma; 3) many bridging structures
between the filaments associated with the sarcolemma
and the extracellular matrix were frequently detected in
the plasma membrane; and 4) compact filaments
associated with the sarcolemma almost disappeared near
the caveolae, and only thin filaments were anchored to
their neck parts. The special arrangement of the
cytoskeletal components, which is probably necessary
for the intestinal motility, characterizes the topographical
difference of the smooth muscle sarcolemma.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.