Improving anticancer therapy with naringenin-loaded silk fibroin nanoparticles

Fuster, Marta G.; Carissimi, Guzmán; García Montalbán, Mercedes; Víllora Cano, Gloria

Publication:
Improving anticancer therapy with naringenin-loaded silk fibroin nanoparticles

dc.contributor.author	Fuster, Marta G.
dc.contributor.author	Carissimi, Guzmán
dc.contributor.author	García Montalbán, Mercedes
dc.contributor.author	Víllora Cano, Gloria
dc.contributor.department	Ingeniería Química
dc.date.accessioned	2025-01-15T09:57:46Z
dc.date.available	2025-01-15T09:57:46Z
dc.date.issued	2020-04-10
dc.description	© 2020 by the authors. his manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published Manuscript version of a Published Work that appeared in final form in Nanomaterials. To access the final edited and published work see https://doi.org/10.3390/nano10040718
dc.description.abstract	Naringenin (NAR), a flavonoid present in a variety of fruits, vegetables and herbs, exhibits a wide range of pharmacological effects, including anticancer activity. Nevertheless, its application in cancer therapy is limited due to its low bioavailability at the tumour site because of its poor solubility in water and slow dissolution rate. To improve the therapeutic efficacy of NAR, emergent research is looking into using nanocarriers. Silk fibroin (SF), from the Bombyx mori silkworm, is a biocompatible and biodegradable polymer with excellent mechanical properties and an amphiphilic chemistry that make it a promising candidate as a controlled release drug system. The aim of this work is to synthesize naringenin-loaded silk fibroin nanoparticles (NAR-SFNs) by dissolving the SF in the ionic liquid 1-ethyl-3-methylimidazolium acetate, using high-power ultrasounds and rapid desolvation in methanol followed by the adsorption of NAR. The NAR-SFNs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy and thermogravimetric analysis. The drug loading content and encapsulation efficiency were calculated. The drug release profile best fitted a first order equation. The cytotoxicity effects of free NAR, bare silk fibroin nanoparticles (SFNs) and NAR-SFNs were assessed on HeLa and EA.hy926 cells via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated the higher in vitro anticancer potential of synthesized NAR-SFNs than that of free NAR in HeLa cancer cells.	es
dc.format	application/pdf	es
dc.identifier.citation	Nanomaterials, 2020, Vol. 10, Issue 4 : 718
dc.identifier.doi	https://doi.org/10.3390/nano10040718
dc.identifier.issn	Electronic: 2079-4991
dc.identifier.uri	http://hdl.handle.net/10201/148450
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	PROYECTO 1 Ámbito del proyecto: nacional Agencia financiadora: Ministerio de Economía y Competitividad Convocatoria: 2017 Nombre del proyecto: PRODUCCIÓN DE NANOESTRUCTURAS FUNCIONALIZADAS BASADAS EN BIOPOLÍMEROS COMO AGENTES ANTICANCERÍGENOS Y ANTIMICROBIANOS Código o número del acuerdo de subvención: CTQ2017-87708-R PROYECTO 2 Ámbito del proyecto: regional Agencia financiadora: Fundación Séneca Convocatoria: 2018 Nombre del proyecto: SÍNTESIS DE NANOMATERIALES AVANZADOS BASADOS EN BIOPOLÍMEROS COMO SISTEMAS DE LIBERACIÓN DE FÁRMACOS Código o número del acuerdo de subvención: Ref. 20977/PI/18 AYUDA Ámbito de la ayuda: nacional Agencia financiadora: Ministerio de Economía y Competitividad Convocatoria: 2018 Nombre de la ayuda: Contrato predoctoral de Formación del Personal Investigador (FPI) Código o número del acuerdo de subvención: PRE2018-086441	es
dc.relation.publisherversion	https://www.mdpi.com/2079-4991/10/4/718
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Anticancer activity	es
dc.subject	Naringenin	es
dc.subject	Silk fibroin	es
dc.subject	Nanoparticle	es
dc.subject	Green synthesis	es
dc.subject	Ionic liquids	es
dc.subject	Cytotoxicity	es
dc.title	Improving anticancer therapy with naringenin-loaded silk fibroin nanoparticles	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es