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In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds

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dc.contributor.authorMoreno Ayuso, Juan Manuel
dc.contributor.authorRamil‑Rego, Pablo
dc.contributor.authorGallego, Pedro Pablo
dc.contributor.authorBarreal, M. Esther
dc.contributor.authorGarcía Pérez, Pascual
dc.contributor.departmentTecnología de Alimentos, Nutrición y Bromatología
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2026-02-26T12:11:20Z
dc.date.available2026-02-26T12:11:20Z
dc.date.copyright© 2019, Springer Nature B.V.
dc.date.issued2019-06-15
dc.description.abstractDifferent Eryngium species have been used with ornamental, agricultural and medicinal purposes, as a consequence of their chemical constituents. In the southwest Europe the endemic Eryngium viviparum, presents a high risk of extinction and ex situ strategies are high recommended for efficient conservation and re-introduction program. The objective of this study was to satisfy a dual objective: (i) to develop an ex situ conservation strategy through micropropagation and (ii) taking advantage of the extraordinary potential of plant tissue culture, produce a considerable amount of plant material to carry out a preliminary phytochemical study, based on the accumulation of phenolic compounds and their associated antioxidant activity. First a factorial design was conducted in order to study the effect of two cytokinins (6- benzylaminopurine, BAP, and kinetin, KIN), at three levels (0, 1 and 2 mg L−1), on shoot multiplication. Later another factorial design was applied, by using three levels of MS medium salt strength (full, half and quarter- strength) and four sucrose levels (0, 1, 2, and 3%) for improving shoot elongation and rooting. In parallel, a preliminary quantification of total phenolic and flavonoid contents from E. viviparum aerial parts was determined. The simple micropropagation protocol designed allowed obtaining a high rates of shoot multiplication (5.1–5.8 new shoots), rooting (100%) with healthy long roots (3.1–3.5 cm) and plantlet acclimatization (96%). Moderate antioxidant activity was recorded in hydromethanolic extracts from E. viviparum aerial parts. High correlation between total phenolic content and BAP levels in the culture media was found. In conclusion, the micropropagation procedure described here for the endangered E. viviparum can be used as new and very efficient ex situ conservation strategy, and as potential source of antioxidants, conferring an added-value to this plant.
dc.formatapplication/pdf
dc.format.extent9
dc.identifier.citationPlant Cell Tiss Organ Cult 138, 427–435 (2019)
dc.identifier.doihttps://doi.org/10.1007/s11240-019-01638-y
dc.identifier.eissn1573-5044
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/10201/214502
dc.languageeng
dc.publisherSpringer
dc.relationThis research was supported by TREMEDAL-Inland wetlands of Northern Iberian Peninsula: management and restoration of mires and wet environments European Union (LIFE11 NAT/ES/000707, 2012-2015). This work was funded by Xunta de Galicia, Spain (CITACA Strategic Partnership, Reference: ED431E 2018/07) and “Red de Uso Sostenible de Recursos y Residuos” (ED431D 2017/18). The authors acknowledge the FPU grant from the Spanish Ministry of Education, Culture and Sport (reference FPU15/04849) to P. García-Pérez.
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s11240-019-01638-y
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAntioxidant activity
dc.subjectEndemic plant
dc.subjectPhenolic and flavonoids content
dc.subjectPhytochemistry
dc.subjectThreatened plant conservation
dc.subjectApiaceae
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleIn vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione9791447-451b-401b-8128-23cc2a8ebf72
relation.isAuthorOfPublication1ac040a7-86d6-47d4-a039-32d437bebf9b
relation.isAuthorOfPublication.latestForDiscoverye9791447-451b-401b-8128-23cc2a8ebf72
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