17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream

Gómez González, Nuria Esther; Cabas, Isabel; Rodenas, María del Carmen; Arizcun, Marta; García Ayala, Alfonsa; Mulero Méndez, Victoriano Francisco

Publication:
17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream

dc.contributor.author	Gómez González, Nuria Esther
dc.contributor.author	Cabas, Isabel
dc.contributor.author	Rodenas, María del Carmen
dc.contributor.author	Arizcun, Marta
dc.contributor.author	García Ayala, Alfonsa
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2021-02-13T19:32:57Z
dc.date.available	2021-02-13T19:32:57Z
dc.date.created	2017
dc.date.issued	2017
dc.description.abstract	17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor. Nevertheless, these previous studies have mainly focused on the head kidney leukocytes and no information exists on peritoneal leukocytes, including mast cells. In the present work, juvenile gilthead seabream fish were fed a pellet diet supplemented with EE2 for 76 days and intraperitoneally injected with hemocyanin plus imject alum adjuvant at the end of EE2 treatment and 92 days later, and the peritoneal immune response was analyzed. EE2 supplementation induced vtg expression but returned to basal levels by 3 months post-treatment. Interestingly, gilthead seabream peritoneal leukocytes express the genes encoding for the nuclear estrogen receptor α and the G protein-coupled estrogen receptor 1 and the dietary intake of EE2 induced these expression. Moreover, EE2 induced an inflammatory response in the peritoneal cavity in unvaccinated fish, which was largely maintained for several months after the cessation of the treatment. However, the impact of EE2 in vaccinated fish was rather minor and transient. Taken together, the study provides fresh information about endocrine immune disruption, focusing on peritoneal leukocytes.	es
dc.format	application/pdf	es
dc.format.extent	18	es
dc.identifier.citation	Developmental and Comparative Immunology, 76, 143-149, 2017
dc.identifier.doi	http:// dx.doi.org/10.1016/j.dd.2017.06.002
dc.identifier.issn	0145-305X
dc.identifier.uri	http://hdl.handle.net/10201/103221
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Ámbito del proyecto (Europeo, nacional o regional): Nacional; regional Agencia/entidad financiadora: Ministerio de Economía y Competitividad; Fundación Séneca (Comunidad Autónoma de la Región de Murcia) Convocatoria: Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad, Convocatoria 2014, Modalidad 1: «Proyectos de I+D+I; Programa de ayudas a las Unidades y Grupos de Excelencia Científica de la Región de Murcia Nombre del proyecto: Análisis de las vías de actuación de contaminantes de origen farmacéutico en la respuesta inmunitaria celular y en la espermatogénesis en peces; Improvement of the Mediterranean aquaculture production by biotechnological tools Código: AGL2014-53167-C3-1-R; 19883 /GERM/15	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución-NoComercial-SinDerivadas 3.0 España	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/es/	*
dc.subject	17α-Ethynylestradiol	es
dc.subject	Peritoneal exudate	es
dc.subject	lmmune response	es
dc.subject	Gilthead seabream	es
dc.subject.other	Ciencias Puras y Naturales	es
dc.title	17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	be292a3e-c75a-428f-ab50-f5dff0b81234
relation.isAuthorOfPublication	369f9ade-8896-4d91-84c5-e3f94a526070
relation.isAuthorOfPublication.latestForDiscovery	be292a3e-c75a-428f-ab50-f5dff0b81234