Publication: MgSO4
treatment preserves the ischemia-induced
reduction in S-100 protein without modification of the
expression of endothelial tight junction molecules
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Date
2009
Authors
Goñi-de-Cerio, Felipe ; Alvarez, Antonia ; Alvarez, Francisco J. ; Rey-Santano, Maria C. ; Alonso-Alconada, Daniel ; Mielgo, Victoria E. ; Gastiasoro, Elena ; Hilario, Enrique
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of this work was to evaluate the
effect of magnesium sulphate (MgSO4
) administration
on blood-brain barrier (BBB) permeabilization after
cerebral hypoxia-ischemia (HI) induced by partial
occlusion of the umbilical cord of premature fetal lambs.
We also characterized BBB dysfunction in terms of the
levels of expression of a panel of BBB proteins;
Occludin, Claudin, Zona Occludens-1, Zonula
Occludens-2, VE-cadherin and ß-catenin. Lambs were
assigned to: Control group: non-injured animals, 0 h
post-partial cord occlusion (0h-PCO) group: animals
subjected to 60 min HI and sacrificed just after the
insult, 3h-PCO group: HI injured animals resuscitated
and managed on ventilation for 3 hours and MgSO4
group: animals which received a dose of 400 mg/kg
MgSO4
after the HI event and managed on ventilation
for 3 hours. Brains were fixed and blocks processed for
S-100 protein immunohistochemistry. Other brains were
dissociated and processed for S-100 and BBB protein
immunochemistry for analysis by flow cytometry. The
percentage of S-100 positive cells was found to be
dramatically reduced in all studied brain tissues in the
3h-PCO group with respect to the other groups. No
differences were found in the percentage or mean
intensity of BBB protein immunolabeled cells among the
groups. In the MgSO4
group, the percentage of S-100
positive cells 3 h after the HI event was similar to the
control group. These results suggest that MgSO4
treatment preserves the ischemia-induced reduction in S-
100 protein without modification in the expression of
endothelial tight junction molecules. We speculate that
MgSO4
treatment confers neuroprotection by restoration
of blood brain permeability in hypoxia-ischemia.
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