Publication:
Hsa_circ_0070440 mediates the prognosis and progress of human prostate cancer

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Huang-40-113-121-2025.pdf(1.93 MB)
Date
2025
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Authors
Huang, Min ; Zhang, Junming ; Luo, Wentao ; Li, Tingting ; Song, Qiong ; Zhang, Lixiao ; Cao, Min ; Li, Shuang
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-765
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info:eu-repo/semantics/article
Description
Abstract
Background. This study was designed to explore whether hsa_circ_0070440 was dysregulated in prostate cancer (PCa), and assess the effects of hsa_circ_0070440 alteration on PCa prognosis and cell function. Methods. The expression levels of hsa_circ_ 0070440 were assessed in PCa tissues and cell lines. After the classification of patients with PCa based on mean hsa_circ_0070440 level in 138 cases, Chi-square test and survival analyses (Kaplan-Meier method and multivariable Cox proportional hazards analysis) were performed to assess the predictive value of hsa_circ_0070440 in treatment failure (TTF), time to PSA progression (TTPP) and overall survival time. To examine the function of hsa_circ_0070440 in PCa cells, 22Rv1 and C4-2B cells were used for CCK-8 proliferation and Transwell migration assays. Hsa_circ_0070440- and TXNDC5-specific bindings with miR-382/383-5p were validated by bioinformatic analysis and luciferase gene reporter assay. Results. An increased expression of hsa_circ_ 0070440 was found in PCA tissues and cell lines, associated with clinical T stage (p=0.021) and lymph node metastasis. Hsa_circ_0070440 predicted poor overall survival, TTPP, and TTF, acting as independent prognostic factors for overall survival, TTPP, and TTF in patients with PCa. Knockdown of hsa_circ_0070440 inhibited cell proliferation and migration in vitro. Furthermore, hsa_circ_0070440 could sponge miR-382/383-5p. TXNDC5 was a common target gene for miR-382/383-5p in PCa cells. Conclusion. This study demonstrated that hsa_circ_0070440 can predict the prognosis of PCa patients. Hsa_circ_0070440 can facilitate the proliferation and migration of PCa cells, possibly by sponging miR-382/383-5p
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Prostate cancer , Hsa_circ_0070440 , Cell function
Citation
Histology and Histopathology Vol. 40, nº01 (2025)
URI
http://hdl.handle.net/10201/148451
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Histology and histopathology Vol.40, nº1 (2025)
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