Publication: Beta-catenin and survivin expression in keratocystic odontogenic tumor (KCOT).
A comparative immunohistochemical study in primary, recurrent and nevoid basal cell
carcinoma syndrome (NBCCS)-associated lesions
Authors
Leonardi, Rosalia ; Matthews, J.B. ; Loreto, Carla ; Musumeci, Giuseppe ; Campisi, G. ; Lo Muzio, L. ; Dos Santos, J.N. ; Pastorino, L. ; Bufo, P. ; Pannone, G.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Aim: To determine the epithelial expression
of ß-catenin and survivin in sporadic (primary, and
recurrent) and nevoid basal cell carcinoma syndrome
(NBCCS) keratocystic odontogenic tumour (KCOT) in
order to assess activation of the ß-catenin pathway and
evidence of apoptotic inhibition, processes that may
contribute to the known differences in their biological
behaviour.
Materials and Methods: Sections from 40 cases of
KCOT (19 sporadic/primary; 9 sporadic/recurrent and 12
NBCCS-associated) were immunohistochemically
stained for ß-catenin and survivin. The extent and
intensity of immunoreactivity within the lining
epithelium was assessed, using semi-quantitative scales,
independently by two pathologists who were blinded to
the clinical-pathological data. Data were analysed using
Kruskal-Wallis test and, for pair-wise comparisons,
Mann-Whitney test with Bonferroni correction.
Results: All cystic epithelial linings stained for ß-
catenin and survivin but there were differences in the
pattern and intensity of staining among KCOT types.
Sporadic primary KCOT showed weaker staining for ß-
catenin (P=0.0003) and survivin (P<0.0048) that was
restricted to the basal and para-basal layers only,
compared to sporadic recurrent and NBCCS-associated
KCOT, which showed expression throughout all
epithelial layers. There were no differences in ß-catenin
expression among recurrent and NBCCS-associated
KCOT, whereas the intensity of survivin staining was
higher in NBCCS-KCOT (P=0.0003). Nuclear staining
for ß-catenin was found exclusively in recurrent (5/9
cases) and NBCCS-associated (4/12 cases) KCOT.
Conclusion: The data demonstrate ß-catenin
delocalization and survivin over-expression in recurrent
sporadic and NBCCS-associated KCOT suggesting that
these pathways are related to apoptotic inhibition have a
role in KCOT growth and recurrence.
Citation
Histology and Histopathology, vol. 28, nº 9 (2013)
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