Chemoreceptors with C-terminal pentapeptides for CheR and CheB binding are
abundant in bacteria that maintain host interactions

Krell, Tino; Ortega Retuerta, Álvaro

Publication:
Chemoreceptors with C-terminal pentapeptides for CheR and CheB binding are abundant in bacteria that maintain host interactions

dc.contributor.author	Krell, Tino
dc.contributor.author	Ortega Retuerta, Álvaro
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2021-11-05T15:18:01Z
dc.date.available	2021-11-05T15:18:01Z
dc.date.issued	2020-07-16
dc.description.abstract	Chemosensory pathways represent a major prokaryotic signal transduction mechanism that is based on signal sensing by chemoreceptors. An essential feature of chemosensory pathways is the CheR and CheB mediated control of chemoreceptor methylation causing pathway adaptation. At their C-terminal extension the Tar and Tsr model chemoreceptors contain a pentapeptide that acts as an additional CheR and CheB binding site. The relevance of this pentapeptide is poorly understood since pentapeptide removal from Tar/Tsr causes receptor inactivation, whereas many other chemoreceptors do not require this pentapeptide for correct function. We report here a bioinformatic analysis of pentapeptide containing chemoreceptors. These receptors were detected in 11 bacterial phyla and represent approximately 10 % of all chemoreceptors. Pentapeptide containing chemoreceptors are mainly found in Gramnegative bacteria, are of low abundance in Gram-positive species and almost absent from archaea. Almost 50 % of TarH ( Tar homologue) ligand binding domain containing chemoreceptors possess pentapeptides, whereas chemoreceptor families with other ligand binding domains are devoid of pentapeptides. The abundance of chemoreceptors with C-terminal pentapeptides correlated negatively with the number of chemoreceptor genes per genome. The consensus sequence reveals a negative net charge for many pentapeptides. Pentapeptide containing chemoreceptors are very abundant in the order Enterobacterales, particularly in the families Pectobacterium and Dickeya, where they represent about 50 % of the total number. In contrast, bacteria with primarily free living lifestyles have a reduced number of pentapeptides such as approximately 1% for Pseudomonadales. It is proposed that pentapeptide function is related to mechanisms that permit host interaction.	es
dc.format	application/pdf	es
dc.format.extent	36	es
dc.identifier.citation	Computational and Structural Biotechnology Journal
dc.identifier.doi	10.1016/j.csbj.2020.07.006
dc.identifier.issn	2001-0370
dc.identifier.uri	http://hdl.handle.net/10201/113768
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Plan Estatal. Ministerio de Economía y Competitividad. Proyecto: BIO2016-76779-P	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S200103702030338X	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	CheR	es
dc.subject	chemosensory pathway	es
dc.subject	chemotaxis	es
dc.subject	chemoreceptor	es
dc.subject	C-terminal pentapeptide	es
dc.subject.other	Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.title	Chemoreceptors with C-terminal pentapeptides for CheR and CheB binding are abundant in bacteria that maintain host interactions	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	12370e68-6859-4506-8ff9-cd5c6b488194
relation.isAuthorOfPublication.latestForDiscovery	12370e68-6859-4506-8ff9-cd5c6b488194