Publication: Abnormalities in dendritic cell and macrophage accumulation in the pancreas of nonobese diabetic (NOD) mice during the early neonatal period
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Date
2002
Authors
Charré, S. ; Rosmalen, J.G.M. ; Pelegri, C. ; Alves, V. ; Leenen, P.J.M. ; Drexhage, H.A. ; Homo-Delarche, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Dendritic cell (DC), macrophage (Mø) and
l y m p h o cyte infiltrations have been observed in normal
human perinatal pancreata, but have never been
investigated so early in control mice. In type 1 diabetesprone
NOD mice, these cells are thought to infiltrate first
the periphery of the islets of Langerhans around weaning
before further islet infiltration and ß-cell destruction.
We quantified, during the first month of life, the
numbers of DC (characterized by CD11c positivity and
dendritic morphology), histiocyte-like Mø (characterized
by ER-MP23 positivity) and Mø with scave n g i n g
potential (characterized by BM8 positivity) in C57BL/6,
DBA/2 and BALB/c control, and NOD and lymphocytedeficient
NODscid mouse pancreata.
First, CD11c+ DC were present at low densities from
birth onwards in control pancreata, while densities were
higher in NOD and NODscid. Second, high numbers of
BM8+ and ER-MP23+ Mø were observed at birth in all
strains investigated. After birth, particularly BM8+ cells
disappeared progressively in control strains, but not in
NOD and NODs c i d. Third, NOD mice also had more
E R - M P 2 3+ Mø at birth compared to controls. Finally,
DC and Mø localizations were similar in all strains, i.e.,
mostly as dispersed cells in periva s c u l a r, periductular,
peri-islet areas and interlobular septa. The most
remarkable finding was that particularly BM8+ Mø, were
seen at sites of islet neogenesis and predominantly at the
duct-islet interface.
Our data showed that different types of APC were
present in the pancreas during postnatal development in
various control mouse strains and some differences were
o b s e r ved in NOD and NOD s c i d mice from birth
onwards.
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