Publication: MicroRNA expression changes in kidney transplant: diagnostic efficacy of miR-150-5p as potential rejection biomarker, pilot study
Authors
Alfaro, Rafael ; Jiménez Coll, Víctor ; El kaaoui El band, Jaouad ; Martínez Banaclocha, Helios ; Galián, José Antonio ; Parrado, Antonio ; Mrowiec, Anna ; Botella, Carmen ; Moya Quiles, María Rosa ; Boix, Francisco ; Peña Moral, Jesús de la ; Minguela, Alfredo ; Llorente, Santiago ; Muro, Manuel ; Legaz Pérez, Isabel
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Publisher
MDPI
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DOI
https://doi.org/10.3390/jcm10132748
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info:eu-repo/semantics/article
Description
© 2021 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Journal of Clinical Medicine. To access the final edited and published work see https://doi.org/10.3390/jcm10132748
Abstract
Background: The kidney allograft biopsy is considered the gold standard for rejection diagnosis but is invasive and could be indeterminate. Several publications point to the role of miRNA expression in suggesting its involvement in the acceptance or rejection of organ transplantation. This study aimed to analyze microRNAs involved in the differentiation and activation of B and T lymphocytes from kidney transplant (KT) patients’ peripheral blood leukocytes to be used as biomarkers of acute renal rejection (AR). Methods: A total of 15 KT patients with and without acute rejection (AR/NAR) were analyzed and quantified by miRNA PCR array. A total of 84 miRNAs related to lymphocyte differentiation and activation B and T were studied. The functions and biological pathways were analyzed to predict the potential targets of differential expressed miRNAs. Results: Six miRNA were increased in the AR group (miR-191-5p, miR-223-3p, miR-346, miR-423-5p, miR-574-3p, and miR-181d) and miR-150-5p was increased in the NAR group. In silico studies showed a total of 2603 target genes for the increased miRNAs in AR, while for the decrease miRNA, a total of 1107 target-potential genes were found. Conclusions: Our results show that KT with AR shows a decrease in miR-150-5p expression compared to NAR, suggesting that the decrease in miR-150-5p could be related to an increased MBD6 whose deregulation could have clinical consequences.
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Citation
Journal of Clinical Medicine 2021, 10(13), 2748
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