Publication: Human articular chondrocytes, synoviocytes and
synovial microvessels express aquaporin water
channels; upregulation of AQP1 in rheumatoid arthritis
Authors
Trujillo, E. ; Gonzalez, T. ; Marín, R. ; Martín-Vasallo, P. ; Marples, D. ; Mobasheri, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Recent studies have shown that aquaporin
water channels are expressed in human Meckel’s
cartilage. The aim of the present investigation was to
determine if human articular chondrocytes and
synoviocytes express aquaporin 1 (AQP1) water
channels and to establish if there are any alterations in
AQP1 expression in osteoarticular disorders such as
osteoarthritis (OA) and rheumatoid arthritis (RA).
Immunohistochemistry was employed semiquantitatively
to compare the expression of AQP1 in
human chondrocytes derived from normal, OA and RA
joints. PCR, cloning and sequencing confirmed the
presence of AQP1 transcripts in chondrocytes. Normal
human tissue microarrays including samples of kidney,
choroid plexus and pancreas were used as positive
controls for AQP1 expression. In most tissues AQP1
was expressed along endothelial barriers. In the kidney
AQP1 was present in the glomerular capillary
endothelium, proximal tubule and descending thin limbs.
AQP1 was also localized to pancreatic ducts and acini
and the apical membrane domain of the choroid plexus.
Immunohistochemistry showed that AQP1 is expressed
in synovial micro-vessels, synoviocytes and
predominantly in chondrocytes located in the deep zone
of articular cartilage. Image analysis of normal, OA and
RA cartilage suggested that AQP1 may be upregulated
in RA. This is the first report of AQP1 mRNA and
protein expression in articular chondrocytes and
synoviocytes. These findings suggest a potential role for
AQP1 and possibly other members of the AQP gene
family in the movement of extracellular matrix and
metabolic water across the membranes of chondrocytes and synoviocytes for the purposes of chondrocyte
volume regulation and synovial homeostasis.
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