Publication: Dynamic assembly of tight junction-associated proteins ZO-1, ZO-2, ZO-3
and occludin during mouse tooth development
Authors
Unda, F. ; Pérez-Nanclares, G. ; Le Morvan, V. ; Hernández, C. ; Vilaxa, A. ; De-la-Fuente, M. ; Gorry, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tight junctions might play a role during
tissue morphogenesis and cell differentiation. In order to
address these questions, we have studied the distribution
pattern of the tight junction-associated proteins ZO-1,
ZO-2, ZO-3 and occludin in the developing mouse tooth
as a model. A specific temporal and spatial distribution
of tight junction-associated proteins during tooth
development was observed. ZO-1 appeared
discontinuously in the cell membrane of enamel organ
and dental mesenchyme cells. However, endothelial cells
of the dental mesenchyme capillaries displayed a
continuous fluorescence at the cell membrane. Inner
dental epithelium first showed an evident signal for ZO-
1 at the basal pole of the cells at bud/cap stage, but ZO-1
was accumulated at the basal and apical pole of
preameloblast/ameloblasts at late bell stage.
Surprisingly, in the incisor ZO-1 decreased as the inner
dental epithelium differentiated, and was re-expressed in
secretory and mature ameloblasts. On the contrary, ZO-2
was confined to continuous cell-cell contacts of the
enamel organ in both molars and incisors. The lateral
cell membrane of inner dental epithelial cells was
specifically ZO-2 labeled. However, ZO-3 was
expressed in oral epithelium whereas dental embryo tissues were negative. In addition, occludin was hardly
detected in dental tissues at the early stage of tooth
development, but was distributed continuously at the cell
membrane of endothelial cells of ED19.5 dental
mesenchyme. In incisors, occludin was detected at the
cell membrane of the secretory pole of ameloblasts. The
occurrence and relation during tooth development of
tight junction proteins ZO-1, ZO-2 and occludin, but not
ZO-3, suggests a combinatory assembly in tooth
morphogenesis and cell differentiation.
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