Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis

Marqués Gálvez, José Eduardo; Morte Gómez, María Asunción; Navarro Ródenas, Alfonso; Pérez Gilabert, Manuela; García Carmona, Francisco

Publication:
Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis

dc.contributor.author	Marqués Gálvez, José Eduardo
dc.contributor.author	Morte Gómez, María Asunción
dc.contributor.author	Navarro Ródenas, Alfonso
dc.contributor.author	Pérez Gilabert, Manuela
dc.contributor.author	García Carmona, Francisco
dc.contributor.department	Biología Vegetal
dc.contributor.other	Facultades de la UMU::Facultad de Biología
dc.date.accessioned	2026-02-20T10:14:23Z
dc.date.available	2026-02-20T10:14:23Z
dc.date.copyright	© 2019 Marqués-Gálvez et al.
dc.date.issued	2019-07-10
dc.description.abstract	Terfezia claveryi Chatin is a mycorrhizal fungus that forms ectendomycorrhizal associations with plants of Helianthemum genus. Its appreciated edibility and drought resistance make this fungus a potential alternative crop in arid and semiarid areas of the Mediterranean region. In order to increase the knowledge about the biology of this fungus in terms of mycorrhiza formation and response to drought stress, a catalase from T. claveryi (TcCAT-1) has been purified to apparent homogeneity and biochemically characterized; in addition, the expression pattern of this enzyme during different stages of T. claveryi biological cycle and under drought stress conditions are reported. The results obtained, together with the phylogenetic analysis and homology modeling, indicate that TcCAT-1 is a homotetramer large subunit size monofunctional-heme catalase belonging to Clade 2. The highest expression of this enzyme occurs in mature mycorrhiza, revealing a possible role in mycorrhiza colonization, but it is not upregulated under drought stress. However, the H2O2 content of mycorrhizal plants submitted to drought stress is lower than in well watered treatments, suggesting that mycorrhization improves the plant’s oxidative stress response, although not via TcCAT-1 upregulation.
dc.format	application/pdf
dc.format.extent	18
dc.identifier.citation	PLoS ONE, 2019, Vol. 14(7): e0219300
dc.identifier.doi	https://doi.org/10.1371/journal.pone.0219300
dc.identifier.eissn	1932-6203
dc.identifier.uri	http://hdl.handle.net/10201/208842
dc.language	eng
dc.publisher	Public Library of Science
dc.relation	This work was supported by Spanish grants from AEI-FEDER, UE (CGL2016-78946-R; AGL2017-86526P) and from Fundación Séneca- Agencia de Ciencia y Tecnología de la Región de Murcia (20866/PI/18 and 19893/GERM/15). ANR holds a postdoctoral contract (IJCI-2016-28252) and JEMG a PhD grant (DI-14-06904) both from MINECO (Ministerio de Economia y Empresa, Gobierno de España). Additionally, Thader Biotechnology SL (https://thaderbiotechnology.com/) provided support in the form of salary to JEMG.
dc.relation.publisherversion	https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219300
dc.rights	Attribution 4.0 International	*
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject.ods	No relacionado con ningún objetivo de desarrollo sostenible
dc.title	Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
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