Publication:
Co-localization of the zinc transporter ZnT8 (slc30A8) with ghrelin and motilin in the gastrointestinal tract of pigs

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Schweiger-31-205-211-2016.pdf(5.25 MB)
Date
2016
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Authors
Schweiger, Markus ; Steffl, Martin ; Amselgruber, Werner M.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-663
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info:eu-repo/semantics/article
Description
Abstract
Zinc is an important co-factor for insulin storage in pancreatic β-cells of different species and the uptake of this ion into insulin containing secretory vesicles is managed by the zinc transporter, ZnT8, a member of the slc30A gene family. Recent studies indicate that this protein is a major autoimmune target in human type 1A diabetes and has also been implicated by genome-wide association studies in type 2 diabetes. Since individuals suffering from type 1 diabetes often develop gastrointestinal motility disorders, we investigated the expression of ZnT8 in the porcine gastrointestinal tract. For this purpose, we studied the cell-type specific expression of ZnT8 in the gut and its co-expression with endocrine hormones that are closely linked to intestinal motility regulation. Nested RT-PCR and immunostaining of sequential serial sections, as well as double-immunostaining using antibodies directed against ZnT8, ghrelin, motilin, neurotensin, serotonin and glucagon-like peptide 1, indicated that ZnT8 is colocalized with ghrelin and motilin. Our findings provide important information about the cell-type specific expression of ZnT8 in the porcine gastrointestinal system. The selective and exclusive expression of ZnT8 in two endocrine cell-types that are engaged in motility functions may be of particular interest for further investigations into type I diabetes-associated gastrointestinal dysfunctions.
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ZnT8 , Motilin , Ghrelin , Zinc , Pig
Citation
Histology and Histopathology, vol.31, nº2, (2016)
URI
http://hdl.handle.net/10201/106382
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Histology and histopathology Vol.31, nº2 (2016)
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