Publication: Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Authors
Vidal, Virginia ; Ortiz Sánchez, Juana ; Díaz, Julia I. ; Zafrilla, B. ; Bonete, M.J. ; Ruiz de Ybáñez Carnero, María del Rocío ; Palacios, M.J. ; Benzal, Jesús ; Valera, Francisco ; Cruz, C. de la ; Bautista, V. ; Machordom, A. ; Barbosa, Andrés ; Motas Guzmán, Miguel
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Cambridge University Press
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DOI
https://doi.org/10.1017/S0022149X15000218
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info:eu-repo/semantics/article
Description
©2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted, version of a Published Work that appeared in final form in Journal of Helminthology. To access the final edited and published work see https://doi.org/10.1017/S0022149X15000218
Abstract
Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed.
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