Publication: Animal in vivo models of EBV-associated lymphoproliferative diseases:
Special references to rabbit models
Authors
Hayashi, K. ; Teramoto, N. ; Akagi, T.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Animal models of human EBV-associated
diseases are essential to elucidate the pathogenesis of
EBV-associated diseases. Here we review those previous
models using EBV or EBV-like herpesviruses and
describe the details on our two newly-developed rabbit
models of lymphoproliferative diseases (LPD) induced
by simian EBV-like viruses. The first is Cynomolgus-
EBV-induced T-cell lymphomas in rabbits inoculated
intravenously (77- 90%) and orally (82- 89%) during 2 -
5 months. EBV-DNA was detected in peripheral blood
by PCR from 2 days after oral inoculation, while anti-
EBV-VCA IgG was raised 3 weeks later. Rabbit
lymphomas and their cell lines contained EBV-DNA and
expressed EBV-encoded RNA-1 (EBER-1). Rabbit
lymphoma cell lines, most of which have specific
chromosomal abnormality, showed tumorigenicity in
nude mice. The second is the first animal model for
EBV-infected T-cell LPD with virus-associated
hemophagocytic syndrome (VAHS), using rabbits
infected with an EBV-like herpesvirus, Herpesvirus
papio (HVP). Rabbits inoculated intravenously with
HVP-producing cells showed increased anti-EBV-VCAIgG
titers, and most (85%) subsequently died of fatal
LPD and VAHS, with bleeding and hepatosplenomegaly,
during 22-105 days. Peroral spray of cell-free HVP
induced viral infection with seroconversion in 3 out of 5
rabbits, with 2 of the 3 infected rabbits dying of LPD with VAHS. Atypical T lymphocytes containing HVPDNA
and expressing EBER-1 were observed in many
organs. Hemophagocytic histiocytosis was observed in
the lymph nodes, spleen, bone marrow, and thymus.
These rabbit models are also useful and inexpensive
alternative experimental model systems for studying the
biology and pathogenesis of EBV, and prophylactic and therapeutic regimens.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.