Publication: Expression of cytoskeletal proteins and
ATPase activity in bovine femoral artery and vein intima
Authors
Trosheva, M. ; Dikranian, K. ; Nikolov, Sp.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Intima1 cells play an important role in the
biology of the vascular wall. Variability in the metabolic
activity of intimal smooth muscle cells (SMC), as well
as the differential expression of cellular cytoskeletal
proteins depend on factors such as degree of
differentiation, aging, atherosclerosis, etc. Myosin
ATPase activity and cytoskeletal proteins were studied in
the intima of bovine femoral arteries and veins of mature
animals. In some arteries the intima was thickened and
two distinct layers - inner elastic hyperplastic (EHL) and
outer, musculo-elastic (MEL) were observed. ATPase
activity was well defined in endothelial cells (EC) as
well as in SMC. However, differential enzymatic
expression was observed in thickened intimas. SMC in
the EHL were ATPase negative, while in the MEL they
were ATPase positive. Al1 EC and SMC in the «normal»
intimas were vimentin positive, desmin and cytokeratin
negative. In vessels with thickened intimas, the EHL
showed intensive vimentin positivity; in the MEL
desmin immunoreactive SMC were numerous as were as
those in the media. Vimentin-positive SMC occupied
their innermost part.
Differences in the expression of ATPase activity and
cytoskeletal proteins is discussed in terms of possible
migration of media1 SMC andlor morphological
modulation observed in vessels with altered vascular
walls.
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