Publication: Expression of ADAMTS-5 in deformed human temporomandibular joint discs
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Date
2008
Authors
Matsumoto, Takashi ; Tojyo, Itaru ; Kiga, Norifumi ; Hiraishi, Yukihiro ; Fujita, Shigeyuki
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Objective: To study the expression of a
disintegrin and metalloproteinase with thrombospondin
motifs 5 (ADAMTS-5) in tissue samples of deformed
human temporomandibular joint (TMJ) discs and cells
obtained from the discs. Materials and methods: Eleven
adult human TMJ discs (nine diseased discs and two
normal discs) were used in this study. The nine diseased
discs were obtained from nine patients with internal
derangement (ID) and osteoarthritis (OA) in the TMJ.
These patients all had anteriorly displaced discs and
deformed mandibular condyles, making conservative
therapy impossible. The tissues were immunohistochemically
stained using ADAMTS-5 antibodies. In
addition, an articular disc cell line from one case was
established by collagenase treatment. The subcultured
cells under both normal and hypoxic conditions (O2:
2%) were incubated for 3, 6, 12 and 24 h after addition
of interleukin-1ß (IL-1ß) (1 ng/mL). Subsequently, the
expression of ADAMTS-5 was examined using reverse
transcription-polymerase chain reaction (RT-PCR).
Results: The control group showed negative reactions on
immunohistochemical staining. The discs extracted from
cases with ID and OA presented positive reactions for
ADAMTS-5. The expression of ADAMTS-5 mRNA
increased under both normoxia and hypoxia with the
addition of IL-1ß, and the peak was observed after 3 h.
Conclusion: These results suggest that ADAMTS-5 is
related to deformation and destruction of human TMJ
discs affected by ID and OA.
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