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What is the sensitivity and exactness of post-mortem diagnostic method for cardiopulmonary nematodes in wild carnivores? towards the gold standard

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dc.contributor.authorRuiz de Ybáñez Carnero, María del Rocío
dc.contributor.authorMartínez-Carrasco Pleite, Carlos
dc.contributor.authorArcenillas Hernández, Irene
dc.contributor.departmentSanidad Animal
dc.date.accessioned2023-12-04T09:02:37Z
dc.date.available2023-12-04T09:02:37Z
dc.date.copyright© 2023 The Author(s)
dc.date.issued2023-03-15
dc.description.abstractCardiopulmonary nematodes cause health and fitness disorders in wild and domestic carnivores. The red fox (Vulpes vulpes) participates in the spread of these shared parasites at the domestic-wildlife interface. This study aimed to evaluate the sensitivity of post-mortem diagnostic method for detecting lungworms in carnivores, and its exactness to estimate the parasite intensity of each nematode species. Cardiorespiratory system of fifty-one foxes were examined through three consecutively methodological steps: first, the tracheobronchial tree, pulmonary arteries and their branches were opened (OT); next, lung parenchyma was immersed in water and squeezed (WS); finally, the parenchyma was artificially digested in a pepsin and chlorhydric acid solution (AD). Eucoleus aerophilus, Angiostrongylus vasorum, Crenosoma vulpis and Metathelazia capsulata were identified. The number of recovered nematodes in each step were 454 (OT), 285 (WS) and 141 (AD). The use of OT and WS helped to improve parasite intensity results and decreased false negative cases. Accordingly, when OT and WS were used together, the sensitivity in the detection of parasitized foxes was 96.1%, while the exactness of parasite intensity was 84%. When AD was performed, although sensitivity does not rise, results were more exact, increasing the total number of detected parasites by 16%. Moreover, AD improved the sensitivity in the detection of A. vasorum and M. capsulata, as well as quantifying more exactly the parasite intensity (92.5% and 92.3% of exactness without AD, respectively). Our study provides valuable information that should be taken into account when planning epidemiological studies based on cardiopulmonary nematode detection in carnivores.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.doihttps://doi.org/10.1007/s11259-023-10084-3
dc.identifier.eisbnVeterinary Research Communications 47:1369-1378es
dc.identifier.eissn1573-7446
dc.identifier.issn0165-7380
dc.identifier.urihttp://hdl.handle.net/10201/136325
dc.languageenges
dc.publisherSpringer Naturees
dc.relationThis work was supported by the Autonomous Community of the Region of Murcia (Spain), through the Regional Programme for the Promotion of Research (Action Plan 2019) of the Seneca Foundation, Science and Technology Agency of the Region of Murcia [grant number 20952/PI/18].es
dc.relation.ispartofProyecto: Papel epidemiológico del zorro como portador de agentes zoonósicos y de otros patógenos compartidos con carnívoros domésticos en ambientes antrópicos de clima mediterráneo semiáridoes
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s11259-023-10084-3
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectArtificial digestiones
dc.subjectLungwormses
dc.subjectPost-mortem diagnostices
dc.subjectRed foxes
dc.subject.odsObjetivo 15: Bosques, desertificación y diversidad biológica
dc.subject.odsObjetivo 3: Salud
dc.titleWhat is the sensitivity and exactness of post-mortem diagnostic method for cardiopulmonary nematodes in wild carnivores? towards the gold standardes
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/aceptedVersión
dspace.entity.typePublicationes
relation.isAuthorOfPublication88a85b71-f36d-4906-98dc-9d9434fe816a
relation.isAuthorOfPublication74bbaf1b-2a73-4ec8-b1c6-429311688d3b
relation.isAuthorOfPublication5a27f7a4-5d1c-4d6b-8578-7a71c3f156b7
relation.isAuthorOfPublication.latestForDiscovery88a85b71-f36d-4906-98dc-9d9434fe816a
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