Publication: Comparative analysis of CD1a, S-100, CD83, and CD11c human dendritic
cells in normal, premalignant, and malignant tissues
Authors
Perez, L. ; Shurin, M.R. ; Kogan, D. ; Tourkova, I.L. ; Shurin, G.V.
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Publisher
Murcia : F. Hernández
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Collins, B.
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A number of antibodies that recognize
human dendritic cells (DC) have been identified. The
main aim of this study was to compare and contrast
different antigen retrieval techniques using both
enzymatic and non-enzymatic treatments in order to
determine the expression and distribution of several DC
markers on formalin-fixed, paraffin-embedded tissues.
Normal human lung, oral epithelial hyperplasia lesions,
oral squamous cell carcinoma, and prostate adenocarcinoma
tissues were evaluated using a panel of DC
specific antibodies. The results of immunohistochemical
staining for CD83, CD1a, CD11c, and S-100 DC
markers were compared following the different antigen
retrieval approaches. The overall best results for the
analysis of tumor-associated DC were obtained with the
enzymatic methods. Protease XXIV digestion was
determined to be essential for detection of S-100 and
CD11c positive DC, whereas trypsin and pepsin were
required for the recognition of CD1a and CD83
expressing tumor-associated DC. These results could be
easily adapted for routine practice and should be useful
for characterization of the DC system in cancer patients
for both diagnostic and prognostic purposes. In addition,
standardized procedures for evaluating different subpopulations of tumor-associated DC should bring
new insights in understanding of DC-tumor cell
interaction.
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