Publication: Organogenesis of the digestive
tract in the white seabream, Diplodus
sargus. Histological and histochemical approaches
Authors
Ortiz-Delgado, J.B. ; Darias, M.J. ; Cañavate, J.P. ; Yúfera, M. ; Sarasquete, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The ontogeny of the digestive tract of the
white seabream, Diplodus sargus during the larval
development up to day 45 post-hatching (dph) has been
studied using histological and histochemical techniques.
The oesophageal goblet cells appeared around 6 dph and
contained neutral and acid mucosubstances
(PAS/diastase-PAS and Alcian Blue pH 2.5 positive
reactions). An incipient stomach can be distinguished
from 2 dph but the first sign of gastric gland
development was detected around 13-15 dph, increasing
in number and size by 22-23 dph. Gastric glands were
concentrated in the cardiac stomach region and they had
a high content of protein rich in tyrosine, arginine and
tryptophan. Acidophilic supranuclear inclusions related
to pynocitosis of proteins, were already observed in the
intestinal cells of the posterior intestine around 4-6 dph
(exogenous feeding) and they were present until 25 dph.
The intestinal mucous cells appeared between 15-18 dph
and contained a mixture of neutral and acid
mucosubstances/glycoconjugates, carboxylated ones
being more abundant than the sulphated ones. The
stomach and gastric glands were fully developed by the
first month of life marking the beginning of digestive
features characteristic of the juvenile stage. Around 4-6
dph, glycogen, proteins and neutral lipids were observed
in the granular cytoplasm of hepatocytes. Strongly
acidophilic zymogen granules were also present, at this
time, in the basophilic cytoplasm of the exocrine
pancreatic acinar cells and contained abundant proteins,
especially rich in arginine, tyrosine and tryptophan.
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