Are denture adhesives safe for oral cells?

López García, Sergio; Pecci Lloret, María Pilar; Guerrero Gironés, Julia; Pecci Lloret, Miguel Ramón; Rodríguez Lozano, Francisco Javier; García Bernal, David

Publication:
Are denture adhesives safe for oral cells?

dc.contributor.author	López García, Sergio
dc.contributor.author	Pecci Lloret, María Pilar
dc.contributor.author	Guerrero Gironés, Julia
dc.contributor.author	Pecci Lloret, Miguel Ramón
dc.contributor.author	Rodríguez Lozano, Francisco Javier
dc.contributor.author	García Bernal, David
dc.contributor.department	Dermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned	2025-01-28T08:24:29Z
dc.date.available	2025-01-28T08:24:29Z
dc.date.issued	2020-07-12
dc.description	© 2020 by the American College of Prosthodontists. This document is the Aceptted version of a Published Work that appeared in final form in Journal of Prosthodontics. To access the final edited and published work see https://doi.org/10.1111/jopr.13226
dc.description.abstract	Purpose: To compare the cytotoxicity of six commercially available denture adhesives on human gingival cells: Poligrip Flavour Free Fixative Cream, Fixodent Pro Duo Protection, Novafix cream, FittyDent, Polident Total Action, and Fixodent ProPlus Duo Protection. Material and Methods: Eluates of denture adhesives were brought into contact with human gingival cells and compared to untreated cells (w/o any dental adhesive elute). Cell toxicity was assessed by measuring cell viability (3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assays), cell morphology (immunofluorescenceassays), induction of apoptosis/necrosis and production of reactive oxygen species (ROS) (flow cytometry assays). In addition, the pH of each sample was determined. Data were analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test. Results: All denture adhesives tested led to a reduction in pH, especially Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection. The cell viability assays showed that Fixodent Pro Duo Protection (1:1 72 hours, p = 3.04 × 10−6; 1:2 72 hours, p = 2.07 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) and Fixodent Pro Plus Duo Protection (1:1 72 hours, p = 2.01 × 10−6; 1:2 72 hours, p = 3.03 × 10−6; 1:4 72 hours, p = 2.02 × 10−6) significantly decreased cell viability at all dilutions. Compared to the control group and the rest of the adhesives, Poligrip Flavour Free Fixative Cream (PFF 1:1 72 hours, p = 2.24 × 10−6; 1:2 72 hours, p = 2.44 × 10−6; 1:4 72 hours, p = 2.04 × 10−6) showed a significantly higher cell viability score at all dilutions. Fixodent Pro Duo Protection and Fixodent Pro Plus Duo Protection, both adhesives containing zinc salts in their composition, were responsible for necrosis, and the number of cells was much reduced, with aberrant morphology and pyknotic nucleus. Finally, Fixodent (1:2, p = 2.04 × 10−6, 1:4, p = 0.00036; 1:2, p = 8.82 × 10−6, 1:4, p = 2.30 × 10−6) products significantly promoted ROS production in gingival cells. Conclusions: The results suggest that denture adhesives containing zinc in their composition could be responsible of the decrease of cell viability, ROS production, aberrant cell morphology, and induction of apoptosis and cell death. However, other possible additional cytotoxic factors must be considered. Thus, more studies are necessary to confirm this hypothesis.	es
dc.format	application/pdf	es
dc.identifier.citation	Journal of Prosthodontics, 2020, Vol. 30(1), pp. 65-70
dc.identifier.doi	https://doi.org/10.1111/jopr.13226
dc.identifier.issn	Print: 1059-941X
dc.identifier.issn	Electronic: 1532-849X
dc.identifier.uri	http://hdl.handle.net/10201/149392
dc.language	eng	es
dc.publisher	Wiley
dc.relation	Este estudio está financiado en parte por el Instituto de Salud Carlos III (ISCIII) a través de la Red Española de Terapia Celular (Ter-Cel), subprograma RETICS del Plan Nacional de I+D+I 2013-2016, proyecto 'RD16/0011/0001', financiado por el ISCIII y cofinanciado por el FEDER.	es
dc.relation.publisherversion	https://onlinelibrary.wiley.com/doi/10.1111/jopr.13226
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Biocompatibility	es
dc.subject	Cytotoxicity
dc.subject	Human gingival cells
dc.title	Are denture adhesives safe for oral cells?	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	2372b160-a380-45dd-835a-6f377fbeaf97
relation.isAuthorOfPublication.latestForDiscovery	2372b160-a380-45dd-835a-6f377fbeaf97