Publication: Quantitative histochemistry of phosphorus in the vestibular gelatinous membrane, an electron probe X-ray microanalytical study
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Date
1993
Authors
López-Escámez, J. A. ; Crespo, P. V. ; Cañizares, F. J. ; Campos, Antonio
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Electron probe X-ray microanalysis was used
to study the phosphorus concentration in the otolithic
gelatinous membrane of the saccule and the utricle with
scanning electron microscopy. The otolithic membranes
were plunge-frozen in liquid N2 and freeze-dried.
Quantitative analysis was carried out with an energy
dispersive detector using the peak-to-background ratio
method and different concentrations of KH2P04 salts
dissolved in dextran solutions.
The otolithic gelatinous membrane consists of a 25-
30 pm-thick layer overlying the cilia of the hair cells.
Elements detected in the gelatinous membrane are : Na,
P, S, CI, K and Ca. Although Student's t-test did not
show significant differences between saccular and
utricular concentrations of phosphorus, the distribution
of this element in the two organs was different.
Regresssion analysis established that the concentrations
of phosphorus in the saccular and utricular gelatinous
membrane were dependent. The regression equation
was: y = 18.02x2 + 133.9 (r = 0.83, P < 0.05) where y is
the concentration of phosphorus in the utricle, and x2 the
concentration of phosphorus in the saccule. The findings
obtained in the present study could be related to
structural differences in organic phosphate residues of
the phosphoproteins associated to collagen, or to
different polyphosphoinositide turnover rates in the cell
membrane.
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