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Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal antiLeishmania antibodies in cutaneous Leishmaniasis

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dc.contributor.authorLopez-Trujillo, Emilio
dc.contributor.authorGonzàlez-Farré, Mònica
dc.contributor.authorPujol, Ramon M.
dc.contributor.authorBellosillo, Beatriz
dc.contributor.authorFisa, Roser
dc.contributor.authorRiera, Cristina
dc.contributor.authorAlcover, Magdalena
dc.contributor.authorBarranco, Carlos
dc.contributor.authorMartin-Ezquerra, Gemma
dc.date.accessioned2023-01-13T10:40:46Z
dc.date.available2023-01-13T10:40:46Z
dc.date.issued2021
dc.description.abstractBackground. Different immunohistochemical markers to detect amastigotes in cutaneous Leishmaniasis have been proposed with variable diagnostic usefulness. Objectives. To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous Leishmaniasis. Materials and methods. Thirty-three skin samples corresponding to PCR confirmed cutaneous Leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results. From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, antiCD1a and anti-Leishmania, was 94% [CI 95%: (79,8%- 99,3%)]; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. identification. Conclusions. Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin – eosin and Giemsa staining.es
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dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 36, nº5 (2021)
dc.identifier.doihttps://doi.org/10.14670/HH-18-324
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/127284
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAmastigotees
dc.subjectCD1aes
dc.subjectImmunohistochemistryes
dc.subjectLeishmaniaes
dc.subjectLeishmaniasises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleDiagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal antiLeishmania antibodies in cutaneous Leishmaniasises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.36, nº5 (2021)