Publication:
The postulated mechanism of the protective effect of ginger on the aspirin induced gastric ulcer: Histological and immunohistochemical studies

Thumbnail Image
Files
Khalil-30-855-864-2015.pdf(4.03 MB)
Date
2015
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Salah Khalil, Mahmoud
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­a
publication.page.editor
publication.page.department
DOI
10.14670/HH-11-592
item.page.type
info:eu-repo/semantics/article
Description
Abstract
There are many available drugs for treating gastric ulcer, but they have various side effects. Ginger is a folk, herbal medicine, which is used for treatment of various diseases including gastric ulcer. This study investigates the possible mechanism of the protective effect of ginger on aspirin induced gastric ulcer. Forty adult male albino rats were randomized into four groups (10 animals per each group) and orally received the followings once daily for 5 days: Group I: 3 ml of 1% carboxymethyl cellulose; Group II: ginger powder (200 mg/kg body weight) suspended in 3 mL of 1% carboxymethylcellulose; Group III: aspirin (400 mg/kg body weight) suspended in 3 ml of 1% carboxymethylcellulose in water. Group IV: ginger and 30 minutes later, received aspirin suspended in 1% carboxymethylcellulose, in similar doses as received in groups II and III. On day 6, rats were sacrificed. The animals were anesthetized and the stomach was removed for the macroscopic, histological (Haematoxylin and Eosin and Periodic Acid Shiff) and immunohistochemical investigations (Bax, inducible nitric oxide synthase and heat shock protein 70). Aspirin induced a significant increase of the macroscopic ulcer score, shed and disrupted epithelium, mucosal hemorrhage, submucosal edema and leukocyte infiltration, loss of the mucus of the mucosal surface significantly increased expression of apoptosis regulator Bax, inducible nitric oxide synthase (iNOS) and heatshock protein 70 (HSP70). Ginger ameliorated the histological changes by reducing Bax and iNOS and increasing HSP70 expressions.
publication.page.subject
Ginger , Aspirin , Gastric ulcer , Histological , Immunohistochemical
Citation
Histology and Histopathology, vol.30, nÂş 7, (2015)
URI
http://hdl.handle.net/10201/96684
item.page.embargo
Collections
Histology and histopathology Vol.30, nÂş7 (2015)
Ir a EstadĂ­sticas