Publication: MALDI mass spectrometry imaging of formalin-fixed
paraffin-embedded tissues in clinical research
Authors
Gorzolka, Karin ; Walch, Axel
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
http://doi.org/10.14670/HH-29.1365
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info:eu-repo/semantics/article
Description
Abstract
The molecular investigation of archived
formalin-fixed, paraffin-embedded (FFPE) tissue
samples provides the chance to obtain molecular patterns
as indicatives for treatment and clinical end points.
MALDI mass spectrometry imaging is capable of
localizing molecules like proteins and peptides in tissue
sections and became a favorite platform for the targeted
and non-targeted approaches, especially in clinical
investigations for biomarker research. In FFPE tissues
the recovery of proteomic information is constrained by
fixation-induced cross-links of proteins. The promising
new insights obtained from FFPE in combination with
the comprehensive patients’ data caused much progress
in the optimization of MS imaging protocols to
investigate FFPE samples. This review presents the past
and current research in MALDI MS imaging of FFPE
tissues, demonstrating the improvement of analyses,
their actual limitations, but also the promising future
perspectives for histopathological and tissue-based
research.
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Citation
Histology and Histopathology, Vol. 29, n.º 11 (2014)
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