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Sodium transport systems in human chondrocytes II. Expression of ENaC, Na+ K+ 2CI- cotransporter and Na+ H+ exchangers in healthy

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dc.contributor.authorTrujillo, E.es
dc.contributor.authorAlvarez de la Rosa, D.es
dc.contributor.authorMobasheri, A.
dc.contributor.authorGonzalez, T.
dc.contributor.authorCanessa, C.M.
dc.contributor.authorMartín Vasallo, P.
dc.date.accessioned2011-02-22T11:10:18Z
dc.date.available2011-02-22T11:10:18Z
dc.date.issued1999
dc.description.abstractIn this article, the second of two, we continue our studies of sodium-dependent transport systems in human cartilage from healthy individuals and with osteoarthritis (OA) and rheumatoid arthritis (RA). We demonstrate the presence of the epithelia1 sodium channel (ENaC), previously undescribed in chondrocytes. This system is composed of three subunits, a, 13 and y. We have shown that the human chondrocytes express at least the a and the l3 subunit of ENaC. The expression of these subunits is altered in arthritic chondrocytes. In RA samples the quantity of a and B is significantly higher than in control samples. On the other hand, ENaC a and B subunits are absent in the chondrocytes of OA cartilage. Human chondrocytes also possess three isoforms of the Na+/H+ exchanger (NHE), NHE1, NHE2 and NHE3. The NHE system is composed of a single protein and is believed to participate in intracellular pH regulation. Furthermore, our studies indicate that at least one isoform of the electroneutral Naf/K+/2C1- cotransporter (NKCC) is present in human chondrocytes. There are no obvious variations in the relative expression of NHE isoforms or NKCC between healthy and arthritic cartilage. Our data suggests that chondrocytes from arthritic cartilage may adapt to changes in their environmental sodium concentration through variations in ENaC protein levels. ENaC is also likely to serve as a major sodium entry mechanism, a process that, along with cytoskeletal proteins, may be part of mechanotransduction in cartilage.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/19252
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectOsteoarthritises
dc.subjectRheumatoid arthritises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleSodium transport systems in human chondrocytes II. Expression of ENaC, Na+ K+ 2CI- cotransporter and Na+ H+ exchangers in healthyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.14, nº 4 (1999)