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Axotomy-induced retinal ganglion cell death in adult mice: quantitative and topographic time course analyses

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dc.contributor.authorGalindo Romero, Caridad
dc.contributor.authorAvilés Trigueros, Marcelino
dc.contributor.authorJiménez López, Manuel
dc.contributor.authorValiente Soriano, Francisco Javier
dc.contributor.authorSalinas Navarro, Manuel Ángel
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorVillegas Pérez, Maria Paz
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.departmentAnatomía Humana y Psicobiología
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T08:15:13Z
dc.date.available2026-01-27T08:15:13Z
dc.date.copyright© 2011 Elsevier Ltd.
dc.date.issued2011-02-24
dc.description.abstractThe fate of retinal ganglion cells after optic nerve injury has been thoroughly described in rat, but not in mice, despite the fact that this species is amply used as a model to study different experimental paradigms that affect retinal ganglion cell population. Here we have analyzed, quantitatively and topographically, the course of mice retinal ganglion cells loss induced by intraorbital nerve transection. To do this, we have doubly identified retinal ganglion cells in all retinas by tracing them from their main retinorecipient area, the superior colliculi, and by their expression of BRN3A (product of Pou4f1 gene). In rat, this transcription factor is expressed by a majority of retinal ganglion cells; however in mice it is not known how many out of the whole population of these neurons express it. Thus, in this work we have assessed, as well, the total population of BRN3A positive retinal ganglion cells. These were automatically quantified in all whole-mounted retinas using a newly developed routine. In control retinas, tracedretinal ganglion cells were automatically quantified, using the previously reported method (SalinasNavarro et al., 2009b). After optic nerve injury, though, traced-retinal ganglion cells had to be manually quantified by retinal sampling and their total population was afterwards inferred. In naïve whole-mounts, the mean ( standard deviation) total number of traced-retinal ganglion cells was 40,437 ( 3196) andofBRN3Apositive ones was 34,697( 1821). Retinal ganglion cell loss was first significant for both markers 5 days post-axotomy and by day 21, the last time point analyzed, only 15% or 12% of traced or BRN3A positive retinal ganglion cells respectively, survived. Isodensity maps showed that, in control retinas, BRN3A and traced-retinal ganglion cells were distributed similarly, being densest in the dorsal retina along the naso-temporal axis. After axotomy the progressive loss of BRN3A positive retinal ganglion cells was diffuse and affected the entire retina. In conclusion, this is the first study assessing the values, in terms of total number and density, of the retinal ganglion cells surviving axotomy from 2 till 21 days post-lesion. Besides, we have demonstrated that BRN3A is expressed by 85.6% of the total retinal ganglion cell population, and because BRN3A positive retinal ganglion cells show the same spatial distribution and temporal course of degeneration than traced ones, BRN3A is a reliable marker to identify, quantify and assess, ex-vivo, retinal ganglion cell loss in this species.
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dc.identifier.citationGalindo-Romero, C., Avilés-Trigueros, M., Jiménez-López, M., Valiente-Soriano, F. J., Salinas-Navarro, M., Nadal-Nicolás, F., ... & Agudo-Barriuso, M. (2011). Axotomy-induced retinal ganglion cell death in adult mice: quantitative and topographic time course analyses. Experimental eye research, 92(5), 377-387.
dc.identifier.doihttps://doi.org/10.1016/j.exer.2011.02.008
dc.identifier.issn0014-4835
dc.identifier.urihttp://hdl.handle.net/10201/194029
dc.languageeng
dc.publisherElsevier
dc.relationThis work was supported by research grants from Fundación Séneca 04446/GERM/07; Spanish Ministry of Education and Science SAF-22010-10385; Spanish Ministry of Science and Innovation and ISCIII-FEDER: PI0/70225; PI10/00187, PI006/0780 and Red Temática de Investigación Cooperativa en Oftalmología RD07/ 0062/0001.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S001448351100042X
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectTracing
dc.subjectBRN3A
dc.subjectSpatial distribution
dc.subjectAutomated quantification
dc.subjectTransection
dc.subjectOptic nerve
dc.subject.odsObjetivo 3: Salud
dc.titleAxotomy-induced retinal ganglion cell death in adult mice: quantitative and topographic time course analyses
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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