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Comparative study of MLPA-FISH to determine DNA copy number alterations in neuroblastic tumors

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dc.contributor.authorVillamón, E.
dc.contributor.authorPiqueras, M.
dc.contributor.authorBerbegall, A.P.
dc.contributor.authorTadeo, I.
dc.contributor.authorCastel, V.
dc.contributor.authorNavarro, S.
dc.contributor.authorNoguera, R.
dc.date.accessioned2016-03-08T17:19:21Z
dc.date.available2016-03-08T17:19:21Z
dc.date.issued2011
dc.description.abstractNeuroblastoma tumor cells show complex combinations of genetic aberrations, and to date many different methods have been used for their detection. To apply genome-wide techniques, such as Multiplex Ligation–dependent Probe Amplification (MLPA), in routine diagnosis their validation is appropriate and necessary. DNA copy number alterations in 129 cases of neuroblastic tumors were detected using MPLA, and the results validated by Fluorescence In Situ Hybridization (FISH) (MYCN gene, 1p36, 11q and 17q). Kappa index values showed very good concordance between the two techniques in detecting homogeneous MYCN amplification (1); 11q deletion (0.908) and 17q gain (0.922). The validation results showed that MLPA is a highly efficient technique for diagnosis based on the genetic aberrations in relevant regions in neuroblastoma, showing a high concordance with FISH.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/48280
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 3 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectNeuroblastomaes
dc.subjectGenetic alterationses
dc.subjectFishes
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleComparative study of MLPA-FISH to determine DNA copy number alterations in neuroblastic tumorses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº3 (2011)