Publication:
Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.

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dc.contributor.authorBaenas, Nieves
dc.contributor.authorPiegholdt, Stefanie
dc.contributor.authorSchloesser, Anke
dc.contributor.authorMoreno, Diego A.
dc.contributor.authorGarcía-Viguera, Cristina
dc.contributor.authorRimbach, Gerald
dc.contributor.authorWagner, Anika E.
dc.contributor.departmentTecnología de Alimentos, Nutrición y Bromatología
dc.date.accessioned2024-01-30T12:29:28Z
dc.date.available2024-01-30T12:29:28Z
dc.date.issued2016-02-18
dc.descriptionItem en revisión. Pendiente de cumplimentar metadatos.
dc.description©<2017>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/cc-by /4.0/ This document is the Published version of a Published Work that appeared in final form in [International Journal of Molecular Sciences]. To access the final edited and published work see [https://doi.org/10.3390/ijms17020251]
dc.description.abstractWe used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellituses
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationInternational Journal of Molecular Sciences, 17 (2), 2016, 251
dc.identifier.doihttps://doi.org/10.3390/ijms17020251
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067 (electrónico)
dc.identifier.urihttp://hdl.handle.net/10201/138165
dc.languageenges
dc.publisherMDPI
dc.relationN. Baenas was funded by a FPU (Formación Profesorado Universitario) grant of the Fellowships Programme from the Spanish Ministry of Education.es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/17/2/251es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBrassicaceae
dc.subjectSulforaphene
dc.subjectRadish
dc.subjectSpargel
dc.subjectEnergy metabolism
dc.titleMetabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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