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Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

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dc.contributor.authorMartínez, Cristina A.
dc.contributor.authorCambra, Josep M.
dc.contributor.authorParrilla, Inmaculada
dc.contributor.authorRoca, Jordi
dc.contributor.authorFerreira-Dias, Graça
dc.contributor.authorPallarés, Francisco J.
dc.contributor.authorLucas, Xiomara
dc.contributor.authorVázquez, Juan M.
dc.contributor.authorMartínez, Emilio A.
dc.contributor.authorGil, María A.
dc.contributor.authorRodríguez-Martínez, Heriberto
dc.contributor.authorCuello, Cristina
dc.contributor.authorÁlvarez-Rodríguez, Manuel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2025-01-23T11:02:06Z
dc.date.available2025-01-23T11:02:06Z
dc.date.issued2019-12-18
dc.description© 2019 Authors This document is the published version of a published work that appeared in final form in Frontiers in Veterinary Science This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0 To access the final edited and published work see: https://doi.org/10.3389/fvets.2019.00465
dc.description.abstractBackground: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, and potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected embryo development and the transcriptional pattern of the pig endometria containing embryos. Materials and Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine infusions of either heterologous pooled SP or Beltsville Thawing Solution (BTS; control) 30 min before AI of semen extended to 10% of homologous SP. Embryos (all sows) and endometrium samples (3 sows/group) were removed during laparotomy 6 days after the infusion of SP or BTS to morphologically evaluate the embryos to determine their developmental stage and to analyze the endometrial transcriptome using microarrays (PORGENE 1.0 ST GeneChip array, Affymetrix) followed by qPCR for further validation. Results: Embryo viability was equal between the groups (~93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others. Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.es
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dc.format.extent16es
dc.identifier.citationFront Vet Sci. 2019 Dec 18 6:465.
dc.identifier.doi10.3389/fvets.2019.00465
dc.identifier.issn2297-1769
dc.identifier.urihttp://hdl.handle.net/10201/149106
dc.languageenges
dc.relationThis study was supported by the Ministry of Economy and Competitiveness (Madrid, Spain)/the European Regional Development Fund (AGL2015-69735-R), the Seneca Foundation, Murcia, Spain (grant number 19892/GERM/15) and the Research Council FORMAS, Stockholm, Sweden (Project 2017-00946). EM, MG, HR-M, and MÁ-R obtained the funding to carry out the study.es
dc.relation.publisherversionhttps://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2019.00465/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectEmbryoes
dc.subjectEndometrium
dc.subjectPig
dc.subjectPreimplantation
dc.subjectSeminal plasma
dc.subjectTranscriptome
dc.titleSeminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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