Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles

Munoz Dávila, M.J.; Yagüe, G; Albert, M.; García-Lucas, T.

Publication:
Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles

dc.contributor.author	Munoz Dávila, M.J.
dc.contributor.author	Yagüe, G
dc.contributor.author	Albert, M.
dc.contributor.author	García-Lucas, T.
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2024-01-30T09:07:32Z
dc.date.available	2024-01-30T09:07:32Z
dc.date.issued	2011-07-28
dc.description	©Springer-Verlag 2011. This document is the Published version of a Published Work that appeared in final form in European Journal of Clinical Microbiology and Infectious Diseases. To access the final edited and published work see https://doi.org/10.1007/s10096-011-1356-1
dc.description.abstract	TheperformanceofVitek2wasevaluatedforthe identification and susceptibility testing of Gram-negative bacilli directly frompositivebloodculturesbottles.Direct inoculation of the positive blood cultureswith theVitek cards ID-GN andAST-NO58was comparedwith the standard inoculationmethod based on the sub-culture of the positive blood culture to agar. A total of 142 blood cultureswereincludedinthestudy;ofthose,119werefrom patients’ clinical samples, while 23 were artificially preparedwith strains showing different mechanisms of resistance. A total of 136 (95.8%) strainswere correctly identified to the species level, only 2 (1.4%) weremisidentifiedand4 (2.8%)werenot identified. Susceptibility results were available for all isolates tested against 17 antibiotics, thus, resulting in a total of 2,414 isolate/antimicrobialcombinations.Theerrorratewas2.8%(67/2,414) overall;0.6%(14/2,414)verymajorerrors, 0.1%(3/2,414) major errorsand2.1%(50/2,414)minor errors.Thedirect method detected 88.5%(22/25) of the strains producing extended-spectrumbeta-lactamases (ESBLs). The susceptibility agreement among the added strains with ESBL, AMPchyperproduction, resistance toceftazidime, carbapenemsandcefepimewasveryhigh.Direct identificationand susceptibility testing gave rapid and reliable results, reducingby24h the turnaround timeof themicrobiology laboratory.
dc.format	application/pdf	es
dc.identifier.citation	Eur J Clin Microbiol Infect Dis. 2012 31:663-669.
dc.identifier.doi	https://doi.org/10.1007/s10096-011-1356-1.
dc.identifier.issn	Print: 0934-9723
dc.identifier.issn	Electronic: 1435-4373
dc.identifier.uri	http://hdl.handle.net/10201/138110
dc.language	eng	es
dc.publisher	Springer
dc.relation	Sin financiación externa a la Universidad	es
dc.rights	info:eu-repo/semantics/embargoedAccess	es
dc.title	Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es