Is the development of ascites in alcoholic liver patients influenced by specific KIR/HLA Gene profiles?

Morales, Raquel; Bolarín, José Miguel; Collados Ros, Aurelia; Pons, José Antonio; Muro, Manuel; Legaz Pérez, Isabel

Publication:
Is the development of ascites in alcoholic liver patients influenced by specific KIR/HLA Gene profiles?

dc.contributor.author	Morales, Raquel
dc.contributor.author	Bolarín, José Miguel
dc.contributor.author	Collados Ros, Aurelia
dc.contributor.author	Pons, José Antonio
dc.contributor.author	Muro, Manuel
dc.contributor.author	Legaz Pérez, Isabel
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2024-07-08T11:34:03Z
dc.date.available	2024-07-08T11:34:03Z
dc.date.issued	2023-08-28
dc.description	© 2023 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Biomedicines. To access the final edited and published work see https://doi.org/10.3390/biomedicines11092405
dc.description.abstract	Decompensated cirrhosis is the most common cause of ascites due to hemodynamic and renal alteration by continuous fluid leakage from the hepatic sinusoids and splanchnic capillaries into the interstitial space. Then, fluid leakage exceeds lymphatic return, leading to progressive fluid accumulation directly into the peritoneal cavity. Alcohol consumption is one of the main risks of developing alcoholic cirrhosis (AC), but not all AC patients develop ascites. Avoiding the development of ascites is crucial, given that it deteriorates prognosis and increases the patient mortality patient. The innate immune system plays a crucial role in cirrhosis through natural killer cells, which are abundant in the liver. The aim of this study was to analyze the KIR/HLA-C genetic profile in AC patients with and without ascites to understand this pathology and find predictive clinical susceptibility biomarkers that can help to establish risks and prevent the development of ascites in AC patients. A total of 281 AC patients with and without ascites were analyzed and compared with 319 healthy controls. Genomic DNA was extracted from peripheral blood in all groups. A PCR-SSO assay was performed for KIR/HLA genotyping analysis. A total of 16 activating and inhibitor KIR genes and their corresponding known ligands, epitopes of HLA-C, and their genotypes were analyzed. According to our analysis, C1 epitopes were statistically significantly decreased in AC patients with and without ascites. When comparing AC patients with ascites and healthy controls, a significant decrease in C1 epitope frequency was also observed. A statistically significant decrease was also found when comparing the C1C2 genotype in AC patients without ascites with controls. In conclusion, the absence of KIR2DL2 and KIR3DL1 genes may be a predisposing factor for the development of ascites in AC patients. The KIR2DS2/KIR2DL2 may could be involved in grade I ascites development, and the presence of the C1+ epitope and the homozygous C2C2 genotype may be protective genetic factors against ascites development in AC patients.	es
dc.format	application/pdf	es
dc.format.extent	18	es
dc.identifier.citation	Biomedicines 2023, 11(9), 2405
dc.identifier.doi	https://doi.org/10.3390/biomedicines11092405
dc.identifier.issn	Electronic: 2227-9059
dc.identifier.uri	http://hdl.handle.net/10201/142910
dc.language	eng	es
dc.publisher	MDPI
dc.relation	Our work was possible thanks to support from Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Economy, and Competitiveness (grant number P19/01194) and co-funding from the European Union through the European Fund of Regional Development (FEDER) with the principle of “A manner to build Europe”.	es
dc.relation.publisherversion	https://www.mdpi.com/2227-9059/11/9/2405
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Alcoholic cirrhosis	es
dc.subject	Ascites	es
dc.subject	Human clinical toxicology	es
dc.subject	Liver transplant	es
dc.subject	KIR/HLA-C genes	es
dc.title	Is the development of ascites in alcoholic liver patients influenced by specific KIR/HLA Gene profiles?	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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relation.isAuthorOfPublication.latestForDiscovery	b83b4b59-2d61-40f0-9108-5c6a6d158295