Publication: Immunohistochemical study of immunological
markers: HLAG, CD16, CD25, CD56 and CD68
in placenta tissues in recurrent pregnancy loss
Authors
Papamitsou, Theodora ; Toskas, Alexandros ; Papadopoulou, Kyriaki ; Sioga, Antonia ; Lakis, Sotirios ; Chatzistamatiou, Maria ; Economou, Zinon ; Adriopoulou, Luisa
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Introduction: Recurrent pregnancy loss
(RPL) of unknown etiology is correlated with
immunological alterations during pregnancy. Normally,
changes in leukocyte subpopulations and HLA
expression take place in pregnant uterus in order to
tolerate the semi-allogenic embryo. Objective: Our
research tries to enlighten the immunological changes
that take place in the uterus of women with recurrent
abortions of unknown etiology during first trimester of
pregnancy. Materials and methods: The miscarriage
group was obtained from 25 women who miscarried
between the ages of 35 to 42 years and controls
consisted of 25 healthy women between the ages of 27 to
39 years, who had electively terminated their
pregnancies during the first trimester of pregnancy. The
abortion was processed and specimens taken were
studied using immunohistochemical methods.
Specimens were taken from decidua basalis and decidua
parietalis. Monoclonal antibodies were used against
HLAG (Human Leukocyte Antigen G), CD68( Cluster of
Differentiation 68), CD56, CD16 and CD25. The results
were statistically analysed with Mann-Whitney test.
Results: HLA-G expression in decidua basalis from
miscarriage group was found to be decreased. CD25+
cell expression was found to be invariable in deciduas
from both groups. CD16+ cell and CD68 + cell
expression was found to be increased in deciduas from
the miscarriage group. CD56+ cell expression was found
to be increased in decidua parietalis from miscarriage
group. Conclusion : Several differences in the
immunological profile of deciduas from RPL group were
observed. Changes in feto-protective HLA-G expression
and a possible implication of macrophages and NK cells
were found.
publication.page.subject
Citation
Histology and Histopathology, vol. 29, nº 8, (2014)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/