Publication: The use of microwave irradiation for immunohistochemistry
Authors
Vitarelli, E. ; Sippelli, G. ; Tuccari, G. ; Barresi, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We developed a rapid immunohistochernical
method using a microwave oven in formalin-fixed,
paraffin-embedded sections from normal and
pathological tissues. The strongest immunoreactivity
was obtained for actin, Ca 125, CEA, pan-cytokeratin,
chromogranin A, EMA, GFAP, thyroglobulin, kappa and
lambda chains. In control tissues, processed with
conventional immunocytochemical procedure, the
reactivity was found to be qualitatively and
quantitatively similar. Dako EPOS kits were also
assayed with good staining intensity, shortening the
original technique to 16 min. Our microwave immunohistochemical
method is simple, rapid and it may be
recommended for use in routine laboratories.
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Citation
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