Publication: Ultrastructural changes induced by a-sarcin in a human pulmonary tumor grown in naked mice
Authors
Mohamed-Ali, H. ; Kolkenbrock, H.J. ; Hecker-Kia, A. ; Hinz, N. ; Ulbrich, N.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
a-Sarcin is a cytotoxic polypeptide produced
by Aspergillus giganteus. It suppresses protein synthesis
in yeast and wheat germ extracts and has a purine-specific
RNase activity. The substance has been tested for its
antitumor properties in a series of induced tumor systems
in mice such as sarcoma and carcinoma among others.
Although some of the in vitro effects of a-Sarcin on
certain cellular components have been elucidated, the
biological effects leading to cellular damage are still
obscure.
In this work we analysed the morphological changes
in tumor cells derived from human pulmonary adenocarcinoma
heterotransplanted and grown in naked mice,
induced shortly (24 hours) after a single intratumoral
injection of U-Sarcin (0.4 mgltumor). The results
obtained were: 1) swelling of mitochondria; 2) cell
necrosis with partial removal of necrotic cells by
phagocytosis; 3) thickening of interlobular connective
tissue; 4) hyperplasia of globet-cell-like clear cells.
The mode of action concerning these cellular changes is
presently uncertain.
In view of the severity of these structural alterations it
seems conceivable that a-Sarcin may enter the cell
undergoing interactions with different intracellular
structures. This would require a selective membrane permeabilization,
perhaps induced upon formation of complexes
with negatively-charged membrane phospholipids
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