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A role for mammalian target of rapamycin -mTOR- pathway in non alcoholic steatohepatitis related-cirrhosis

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dc.contributor.authorKubrusly, Márcia Saldanhaes
dc.contributor.authorCorrêa-Giannella, Maria Lúciaes
dc.contributor.authorBellodi-Privato, Marta
dc.contributor.authorde Sá, Sandra Valéria
dc.contributor.authorCauduro Soares, Iberê
dc.contributor.authorWakamatsu, Alda
dc.contributor.authorAvancini Ferreira Alves, Venâncio
dc.contributor.authorGiannella-Neto, Daniel
dc.contributor.authorBacchella, Telesforo
dc.contributor.authorCerqueira Cesar Machado, Marcel
dc.contributor.authorCarneiro D’Albuquerque, Luiz Augusto
dc.contributor.authorPinto Marques Souza de Oliveira, Claudia
dc.date.accessioned2015-09-29T07:18:18Z
dc.date.available2015-09-29T07:18:18Z
dc.date.issued2010
dc.description.abstractSummary. Non-alcoholic fatty liver disease (NAFLD) encompasses the whole spectrum of steatosis, nonalcoholic steatohepatitis (NASH), and NASH-related cirrhosis (NASH/Cir). Although molecular advances have been made in this field, the pathogenesis of NAFLD is not completely understood. The gene expression profiling associated to NASH/Cir was assessed, in an attempt to better characterize the pathways involved in its etiopathogenesis. Methods: In the first step, we used cDNA microarray to evaluate the gene expression profiles in normal liver (n=3) and NASH/Cir samples (n=3) by GeneSifter™ analysis to identify differentially expressed genes and biological pathways. Second, tissue microarray was used to determine immunohistochemical expression of phosphorylated mTOR and 4E-BP1 in 11 normal liver samples, 10 NASH/Cir samples and in 37 samples of cirrhosis of other etiologies to further explore the involvement of the mTOR pathway evidenced by the gene expression analysis. Results: 138 and 106 genes were, respectively, up and down regulated in NASH/Cir in comparison to normal liver. Among the 9 pathways identified as significantly modulated in NASH/Cir, the participation of the mTOR pathway was confirmed, since expression of cytoplasmic and membrane phosphomTOR were higher in NASH/Cir in comparison to cirrhosis of other etiologies and to normal liver. Conclusions: Recent findings have suggested a role for the cellular “nutrient sensor” mTOR in NAFLD and the present study corroborates the participation of this pathway in NASH/Cir. Phospho-mTOR evaluation might be of clinical utility as a potential marker for identification of NASH/Cir in cases mistakenly considered as cryptogenic cirrhosis owing to paucity of clinical data.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/46342
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathology (2010) vol. 25es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCirrhosises
dc.subjectNon alcoholic steatohepatitises
dc.subjectGene expression profile
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleA role for mammalian target of rapamycin -mTOR- pathway in non alcoholic steatohepatitis related-cirrhosises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.25, nº9 (2010)