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Gene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical trial

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dc.contributor.authorNuñez-Sánchez, María A.
dc.contributor.authorGonzález Sarrías, Antonio
dc.contributor.authorGarcía Villalba, Rocío
dc.contributor.authorMonedero Saiz, Tamara
dc.contributor.authorGarcía Talavera, Noelia V.
dc.contributor.authorGómez Sánchez, María B.
dc.contributor.authorSánchez Álvarez, Carmen
dc.contributor.authorGarcía Albert, Ana M.
dc.contributor.authorRodríguez Gil, Francisco J.
dc.contributor.authorRuiz Marín, Miguel
dc.contributor.authorPastor Quirante, Francisco A.
dc.contributor.authorMartínez Díaz, Francisco
dc.contributor.authorTomás Barberán, Francisco A.
dc.contributor.authorEspín, Juan Carlos
dc.contributor.authorGarcía Conesa, María Teresa
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.date.accessioned2024-12-12T11:42:07Z
dc.date.available2024-12-12T11:42:07Z
dc.date.issued2017-01-27
dc.description© 2017 Elsevier Inc. All rights reserved. This document is the Published version of a Published Work that appeared in final form in The Journal of Nutritional Biochemistry (JNB). To access the final edited and published work see https://doi.org/10.1016/j.jnutbio.2017.01.014
dc.description.abstractThe clinical evidence of dietary polyphenols as colorectal cancer (CRC) chemopreventive compounds is very weak. Verification in humans of tissue-specific molecular regulation by the intake of polyphenols requires complex clinical trials that allow for the procurement of sufficient pre- and postsupplementation tissue samples. Ellagitannins (ETs), ellagic acid (EA) and their gut microbiota-derived metabolites, the urolithins, modify gene expression in colon normal and cancer cultured cells. We conducted here the first clinical trial with 35 CRC patients daily supplemented with 900 mg of an ET-containing pomegranate extract (PE) and evaluated the expression of various CRC-related genes in normal and cancerous colon tissues before (biopsies) and after (surgical specimens) 5–35 days of supplementation. Tissues were also obtained from 10 control patients (no supplementation) that confirmed a large, gene- and tissue-specific interindividual variability and impact of the experimental protocol on gene expression, with some genes induced (MYC, CD44, CDKN1A, CTNNB1), some repressed (CASP3) and others not affected (KRAS). Despite these issues, the consumption of the PE was significantly associated with a counterbalance effect in the expression of CD44, CTNNB1, CDKN1A, EGFR and TYMs, suggesting that the intake of this PE modulated the impact of the protocol on gene expression in a gene- and tissue-specific manner. These effects were not associated with the individuals' capacity to produce specific urolithins (i.e., metabotypes) or the levels of urolithins and EA in the colon tissues and did not reproduce in vitro effects evidencing the difficulty of demonstrating in vivo the in vitro results.es
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dc.format.extent8es
dc.identifier.citationJournal of Nutritional Biochemistry 42 (2017) 126–133
dc.identifier.doihttps://doi.org/10.1016/j.jnutbio.2017.01.014
dc.identifier.issnPrint: 0955-2863
dc.identifier.issnElectronic: 1873-4847
dc.identifier.urihttp://hdl.handle.net/10201/147363
dc.languageenges
dc.publisherElsevier
dc.relationThis research was supported by the Projects CICYT-AGL2011- 22447 (MINECO, Spain) and 201370E068 (CSIC, Spain) and partially funded by the “Fundación Séneca de la Región de Murcia” Grupo de Excelencia 19900/GERM/15. M.A.N.S. and A.G.S. are holders of an FPI grant and a “Juan de la Cierva” contract, respectively, from MINECO (Spain). M.B.G.S. and N.V.G.T. are holders of a contract from the Foundation for Education and Health Research in the Region of Murcia (FFIS) (Murcia, Spain). A.G.S., F.A.T.B., J.C.E. and M.T.G.C. are participants to the European COST Action FA1403 POSITIVe: ‘Interindividual variation in response to consumption of plant food bioactives and determinants involved’.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0955286316306775?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectClinical triales
dc.subjectGene expressiones
dc.subjectColon tissueses
dc.subjectEllagitanninses
dc.subjectInterindividual variabilityes
dc.subjectUrolithinses
dc.subjectPomegranate extractes
dc.titleGene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical triales
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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