Binge ethanol and MDMA combination exacerbates HSP27 and Trx-1 (biomarkers of toxic cardiac effects) expression in right ventricle

Navarro-Zaragoza, Javier; Ros-Simó, Clara; Valverde, Olga; Laorden Carrasco, María Luisa; Milanés Maquilón, María Victoria

Publication:
Binge ethanol and MDMA combination exacerbates HSP27 and Trx-1 (biomarkers of toxic cardiac effects) expression in right ventricle

dc.contributor.author	Navarro-Zaragoza, Javier
dc.contributor.author	Ros-Simó, Clara
dc.contributor.author	Valverde, Olga
dc.contributor.author	Laorden Carrasco, María Luisa
dc.contributor.author	Milanés Maquilón, María Victoria
dc.contributor.department	Farmacología
dc.date.accessioned	2021-03-25T18:28:13Z
dc.date.available	2021-03-25T18:28:13Z
dc.date.issued	2019-01
dc.description.abstract	Aims Oxidative stress caused by exposure to drugs of abuse such as ethanol or 3, 4 methylenedioxymethamphetamine (MDMA) may derive from direct or indirect effects in many organs including the heart. The aim of the present work was to evaluate cardiac sympathetic activity and the expression and activation of two antioxidant proteins: heat shock protein27 (HSP27) and thioredoxin-1 (Trx-1) after voluntary binge ethanol consumption, alone and in combination with MDMA. Material and Methods Adolescent mice received MDMA, ethanol or both. Drinking in the dark (DID) procedure was used as a model of binge. HSP27 expression and phosphorylation at serine 82 (pHSP27), Trx-1 expression, tyrosine hydroxylase (TH) and TH phosphorylated at serine 31 (pTH) were evaluated in adolescent mice 48h and 7 days after treatments in the right ventricle. TH, HSP27 expression and phosphorylation and Trx-1 expression were measured by quantitative blot immunolabeling using specific antibodies. Key Findings The expression of HSP27, pHSP27, Trx-1, total TH and pTH in the right ventricle was increased after binge ethanol or MDMA alone. In addition, the combination of binge ethanol+MDMA enhanced TH expression and phosphorylation versus their individual administration. Significance These results indicate that this combination could produce higher activation of sympathetic pathways, which could trigger an increased cell stress. On the other hand, increased HSP27, 3 pHSP27 and Trx-1 expression in the right ventricle by ethanol+MDMA could be a protective mechanism to reduce the adverse effects of oxidative stress caused by both drugs of abuse.	es
dc.format	application/pdf	es
dc.format.extent	29	es
dc.identifier.citation	Life Sciences. 2019 Mar 1 220:50-57
dc.identifier.doi	10.1016/j.lfs.2019.01.050.
dc.identifier.uri	http://hdl.handle.net/10201/105632
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Spanish Ministry of Economy and Innovation and FEDER (SAF/FEDER 2013-49076-P, SAF2017-85679-R and SAF2013-41761-R), the Spanish Ministry of Health, Social Affairs and Equality (PNSD 2014-020) and Instituto de Salud Carlos III (Red Temática de Investigación Cooperativa en Salud -ISCIII-FEDER- RETIC-Trastornos Adictivos RTA, RD12/0028/0003 and RD12/0028/0024).	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	binge ethanol	es
dc.subject	MDMA	es
dc.subject	addiction	es
dc.subject	HSP	es
dc.subject	cardiac	es
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina::615 - Farmacología. Terapéutica. Toxicología. Radiología	es
dc.title	Binge ethanol and MDMA combination exacerbates HSP27 and Trx-1 (biomarkers of toxic cardiac effects) expression in right ventricle	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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