Publication: lmmunocytochemical localization of the vacuolar H+-ATPase pump in the kidney
Loading...
Date
1997
Authors
Bastani, B.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
In this article we review immunocytochemical
localization studies using a monoclonal
antibody raised against the 31 kD subunit of bovine H+-
ATPase, and indirect immunofluorescent staining. In the
proximal tubules there is intense H+-ATPase staining
along the brush borders of S1 and S2, and linear
subvillar invagination staining in SI, S2, and S3
segments. In the thick ascending limb of the loop of
Henle there is a mild to moderate degree apical
cytoplasmic vesicular staining. In dista1 convoluted
tubule there is mild to moderate degree of H+-ATPase
staining which is sharply delineated along the luminal
plasma membrane. In the connecting tubule, the
connecting tubule cells show mild to moderate luminal
membrane and apical cytoplasmic vesicular staining, and
the intercalated cells demonstrate prominent H+-ATPase
staining which is polarized to either apical or basolateral
pole or distributed diffusely throughout the cell. In the
cortical collecting duct the principal cells show minimal
or no staining while the intercalated cells show very
bright H+-ATPase staining with 6 identifiable
morphblogic subtypes based on polarization of the pump
to apical or basolateral poles, and the degree of
polarization (well polarized or poorly polarized). In the
medullary collecting duct the principal cells show no
staining and the intercalated cells show prominent H+-
ATPase staining only in the apical pole. We also describe
adaptive responses to different physiologic manipulations
e.g. chronic oral acid loads, chronic respiratory
acidosis, remnant kidney model, chronic desoxycorticosterone
(DOCA) administration, and chronic
potassium depletion diet. Moreover, we compare the
immunocytochemical localization of the H+-ATPase
pump of rabbit and kidneys.
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.