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Human skin model for mimic dermal studies in pathology with a clinical implication in pressure ulcers

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dc.contributor.authorCristóbal, Lara
dc.contributor.authorOrtega, Miguel A.
dc.contributor.authorAsúnsolo, Ángel
dc.contributor.authorRomero, Beatriz
dc.contributor.authorÁlvarez Mon, Melchor
dc.contributor.authorBuján, Julia
dc.contributor.authorMaldonado, Andrés A.
dc.contributor.authorGarcía Honduvilla, Natalio
dc.date.accessioned2022-05-19T08:24:16Z
dc.date.available2022-05-19T08:24:16Z
dc.date.issued2018
dc.description.abstractDespite advances in regenerative medicine and tissue engineering, human skin substitutes remain a clear goal to achieve. Autografts remain the principal clinical option. The long-term changes in dermis, as well as its response after injuries, are not well known. Research in this field has been hindered by a lack of experimental animal models. This study analyzes the architectural dermal scaffold (collagen and elastin fibers plus fibrillin-microfibrils) changes in a model of human skin pressure ulcers in mice. Immunosuppressed NOD/Scid mice (n=10) were engrafted with human skin of dimensions 4x3 cm. After 60 days as a permanent graft, a pressure ulcer (PU) was created in the human skin using a compression device. Three study groups were established: full-thickness skin graft before (hFTSG) and after applying mechanical pressure (hFTSG-PU). Native human skin was used as control group. Evaluations were conducted with visual and histological assessment. Scaffold components from each group were compared by immunohistochemical staining (tropoelastin, collagen I and III, metalloproteins (MMP), fibulins, and lysil oxidases (LOX) among others). The long-term engrafted skin showed a certain degradative state of dermis scaffold, as noticed by the active expression of MMPs and tropoelastin compared to native skin. However, a great reparative response after pressure ulcer onto the engrafted skin was observed. A significant increase of fibrillin microfibrils components (TGF-β, MAGP-1 and fibrillin-1), and matrix suprastructures of collagen I, III and LOX lead to an active restructuration of dermal tissue. Our human skin model in mice revealed the important role of the dermal scaffold component to reach skin stability and its capability to react to mechanical pressure injuries. These results showed the important role of dermal scaffold to support the histoarchitecture and mechanosensation of the human skin.es
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology, Vol.33, nº9, (2018)
dc.identifier.doiDOI: 10.14670/HH-11-990
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/120237
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHuman skin graftes
dc.subjectPressure ulcerses
dc.subjectWound healinges
dc.subjectDermal scaffoldes
dc.subjectFibrillin-microfibrils componentses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHuman skin model for mimic dermal studies in pathology with a clinical implication in pressure ulcerses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.33, nº9 (2018)