Publication: CircTADA2A Up-regulates MAPK8 by targeting MiR-214-3p and recruiting EIF4A3 to promote the invasion and migration of non-small cell lung cancer cells
Authors
Zhang, Xi ; Lei, Guangyan ; Zhao, Kun ; Zhang, Xinwei ; Dang, Chengxue
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
https://doi.org/10.14670/HH-18-529
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info:eu-repo/semantics/article
Description
Abstract
Background. Non-small cell lung cancer
(NSCLC) occupies 87% of all lung cancer cases. Due to
delayed diagnosis, the prognosis of NSCLC is
unfavorable. To improve the survival of patients with
NSCLC, more effective therapeutic targets urgently need
to be identified. Recently, circular RNAs (circRNAs)
have been revealed to play a crucial role in NSCLC
progression.
Purpose. This research focused on the influence of
circTADA2A on the malignant phenotype of NSCLC
cells and its in-depth regulatory mechanisms.
Methods. RT-qPCR and western blot assays were
done to examine the level of gene/protein of interest.
Wound healing and transwell assays were conducted to
monitor the migration and invasion of NSCLC cells.
Bioinformatics tools and mechanistic assays were
utilized to delve into the underlying mechanism of
circTADA2A in NSCLC cells.
Results. The results demonstrated that circTADA2A
presented a high expression in NSCLC. CircTADA2A
knockdown was revealed to hamper migration and
invasion of NSCLC cells. Mechanistically, circTADA2A
elevated MAPK8 expression through sequestering miR214-3p and recruiting EIF4A3.
Conclusion. CircTADA2A enhances MAPK8
expression by serving as a miR-214-3p sponge and
EIF4A3 decoy, consequently promoting invasion and
migration of NSCLC cells.
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Citation
Histology and Histopathology, Vol.38, nÂş7, (2023)
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