Publication: Expression of transforming growth factor-B3 -GF-B3- in the porcine ovary during the oestrus cycle
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Date
2008
Authors
Steffl, M. ; Schweiger, M. ; Amselgruber, W.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Transforming growth factor-ß (TGF-ß)
proteins are growth factors that have been shown to be
involved in regulation of ovarian follicular development.
Ovarian expression, activity and functional significance
of TGF-ß1 and TGF-ß2 isoforms were extensively
studied in most species. However, little is known about
the biological role of TGF-ß3 previously shown to be
expressed independently of the other two isoforms.
Therefore, expression of TGF-ß3 mRNA and protein
was evaluated by RT-PCR and immunohistochemistry,
respectively, in porcine ovaries collected during different
phases of the oestrus cycle. Results of RT-PCR analysis
showed that TGF-ß3 mRNA is expressed throughout the
oestrus cycle. The level of TGF-ß3 mRNA expression
was found to be higher at metoestrus and dioestrus.
Weak TGF-ß3 immunoreactivity was present in
follicular epithelial cells and oocytes of preantral
follicles in all stages examined. TGF-ß3 protein
expression was exclusively present in theca interna cell
layer of antral follicles, and was particularly prominent
in large antral follicles. Immediately after ovulation,
almost all theca cells outside of the granulosa cell layer
were intensively stained with anti-TGF-ß3. Immunostaining of TGF-ß3 in theca lutein cells rapidly
decreased during corpus luteum development. It is
suggested that TGF-ß3 may play an important role in
modulating theca cell function of pre- and postovulatory
follicles of the pig.
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