Publication: The kinetic differences between sodium nitrite, amyl nitrite and nitroglycerin oxidation of hemoglobin
Loading...
Date
1986
Authors
Tarburton, John Philip ; Metcalf, William K.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The effect of sodium nitrite, amyl nitrite and
nitroglycerin (glyceryl trinitrate) on the hemoglobin of adult
erythrocytes was examined in vitro. Both amyl nitrite and
nitroglycerin reacted immediately with oxyhemoglobin to
effect oxidation into methemoglobin while sodium nitrite
required an inductionary period (lag phase) prior to the
reaction. Kinetic studies of the biniolecular rate law for each
of the preceeding reaction's reactionary periods (log phases)
allowed rate constant calculations to be made. The values
are 1.14 x 10JM niin I, 7.45 x IOJbl lmin I , and 3.50 x
10IM ' m i n 1 for sodium nitrite, amyl nitrite and
nitroglycerin, respectic ely. A comparison of the amyl nitrite
and nitroglycerin rate constants reveals that aniyl nitrite is
approximately 2000-fold more toxic to oxyhemoglobin than
nitroglycerin. These oxidant's effect on in !/itro hemoglobin
solutions are comparable since both reactions approximate
to rectangular hyperbolae. Sodium nitrite reacts about
300-fold faster with oxyhemoglobin than does nitroglycerin.
However, the sodium nitrite reaction proceeds in a
sigmoidal fashion which makes a strict comparison between
these compounds relative toxicities less clear cut.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.