Immunohistochemistry as a tool to characterize human skin wounds of hanging marks

Pérez Cárceles, María Dolores; Giménez, M.; Martínez Díaz, F.; Osuna, Eduardo; Luna, Aurelio; Legaz Pérez, Isabel

Publication:
Immunohistochemistry as a tool to characterize human skin wounds of hanging marks

dc.contributor.author	Pérez Cárceles, María Dolores
dc.contributor.author	Giménez, M.
dc.contributor.author	Martínez Díaz, F.
dc.contributor.author	Osuna, Eduardo
dc.contributor.author	Luna, Aurelio
dc.contributor.author	Legaz Pérez, Isabel
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2024-07-12T10:42:03Z
dc.date.available	2024-07-12T10:42:03Z
dc.date.issued	2019-03
dc.description	© 2018 Romanian Society of Legal Medicine. This document is the Published version of a Published Work that appeared in final form in Romanian Journal of Legal Medicine. To access the final edited and published work see https://doi.org/10.4323/rjlm.2018.354
dc.description.abstract	Estimation of age and vitality of human skin wounds both in the living and dead is essential in forensic practice. The use of immunohistochemical parameters for the age estimation and vitality of human skin wounds remains difficult. Forensic literature describes different biomarkers and methods for the differential diagnosis of vital and post-mortem wounds, but to this day it is unclear its utility. The aim of this study was analysed wounds vital origin in a series of suicidal hangings were vitality had been demonstrated using fibronectin, cathepsin D and P-selectin, in order to discover the morphological changes that occur in a vital wound, and consequently, find useful vital injury diagnosis markers. A total of 15 human vital skin wounds from ligature marks from deaths by suicidal hanging and skin controls from same cadaver were analyzed in a postmortem interval between 19-36 hours. Fibronectine, cathepsin D and P-selectin were detected by immunohistochemistry. Our result shows a strongly fibronectin-positive reaction in basement membranes and interstitial connective tissue in all specimen of wounds of ligature mark. Granular staining pattern characteristic of cathepsin D was observed mainly in the basal layer of the epidermis in normal and wound skin. Cathepsin D analysis in ligature mark showed moderate positive and strong positive cells. A weak positive immunoreactivity P-selectin were found in vital wound compared with undamaged skin. In conclusion, our data show an increase of fibronectin and cathepsin D immunoreactivity expression and a decrease of P-selectin immunoreactivity in skin wounds from ligature marks from deaths by suicidal hanging of a postmortem interval between 19-36 hours.	es
dc.format	application/pdf	es
dc.format.extent	5	es
dc.identifier.citation	Rom J Leg Med 26(4) 354-358 (2018)
dc.identifier.doi	https://doi.org/10.4323/rjlm.2018.354
dc.identifier.issn	Print: 1221-8618
dc.identifier.issn	Electronic: 1844-8585
dc.identifier.uri	http://hdl.handle.net/10201/143064
dc.language	eng	es
dc.publisher	Romanian Society of Legal Medicine
dc.relation	Sin financiación externa a la Universidad	es
dc.relation.publisherversion	https://www.rjlm.ro/index.php/arhiv/680
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Cathepsin D	es
dc.subject	Fibronectin	es
dc.subject	P-selectin	es
dc.subject	Skin wounds	es
dc.subject	Suicidal hanging	es
dc.title	Immunohistochemistry as a tool to characterize human skin wounds of hanging marks	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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