Publication: Heterogeneous ulmtrastrucmtuorf em elanosome
formation in the goldfish induced by osmotic stress
Authors
Schraerrneyer, U. ; Dohms, M. ; Rack, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this study, melanophore cytodifferentiation
in the fins of xanthic goldfish that had
been exposed to osmotic stress for 18 days was
investigated. It was found that multi-vesicular bodies
(MVB) are not the only type of premelanosome.
Granules having a homogeneous matrix also function as
premelanosomes.
The presence of acid phosphatase reaction product
inside the melanin granules indicated that these
organelles in this animal were also related to lysosomes.
DOPA-oxidase of tyrosinase, the key enzyme in
melanogenesis, was surprisingly not only detected in
melanocytes but also in the Golgi stacks of dermal cells.
Due to the mechanisms of premelanosome formation
it is evident that cytoplasmic material also serves as
substrate for melanogenesis.
EDX microanalysis was performed to measure the
ionic composition of the melanin granules. After
aldehyde fixation the newly-formed melanin granules
did not contain Na, but had accumulated Ca.
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