Publication: Effects of diabetes on oxidative stress, periodontal ligament fiber orientation and matrix metalloproteinase 8 and 9 expression during orthodontic tooth movement
| dc.contributor.author | Vicente, Ascensión | |
| dc.contributor.author | Bravo-González, Luis-Alberto | |
| dc.contributor.author | Camacho-Alonso, Fabio | |
| dc.contributor.author | Navarro Cámara, José Antonio | |
| dc.contributor.author | Buendía Marín, Antonio Julián | |
| dc.contributor.department | Dermatología, Estomatología, Radiología y Medicina Física | |
| dc.date.accessioned | 2024-01-25T17:12:39Z | |
| dc.date.available | 2024-01-25T17:12:39Z | |
| dc.date.issued | 2021 | |
| dc.description | Al comprobar la política de acceso abierto de la revista donde está publicado, vemos que no se puede depositar la versión del editor (que es la que nos ha enviado) si antes no se ha pagado una tasa a la revista; sí sería posible depositar la versión aceptada | es |
| dc.description.abstract | Objectives: To evaluate the influence of diabetes on oxidative stress, periodontal ligament (PDL) orientation, and matrix metalloproteinase (MMP) 8 and 9 expressions during orthodontic tooth movement in a rat model. Materials and methods: An orthodontic appliance was placed in 60 Sprague-Dawley rats divided into three groups: normoglycemics (n = 20) and two streptozotocin-induced diabetic groups, one untreated (n = 20) and one insulin-treated (n =20). At 24, 48, and 72 h and 1 week, rats were sacrificed. At each time point, myeloperoxidase (MPO) and malondialdehyde (MDA) were quantified by spectrophotometry, tooth movement was evaluated by micro-CT analysis, and hematoxylin and eosin staining was used to evaluate PDL fiber orientation and immunohistochemistry staining with semi-quantitative H-score analysis of MMP-8 and MMP-9 was performed. Results: At 24 h, MPO activity was significantly higher in untreated-diabetics than normoglycemics. At 24 and 48 h, the MDA level in untreated-diabetic rats was significantly higher than in normoglycemics and insulin-treated animals. At 72 h and 1 week, PDL fibers were oriented significantly more irregularly in untreated-diabetics than in normoglycemics. At all time points,MMP-8 and MMP-9 expressions were significantly higher in both diabetic groups than in the normoglycemic group. After the second day, tooth movement was significantly greater in untreated-diabetics than in the insulin-treated and normoglycemic groups. Conclusions: Mechanical stress in untreated-diabetic rats produces more inflammatory response, oxidative stress, tooth movement, PDL disorganization, and MMP-8 and MMP-9 expressions than among normoglycemics. Insulin reverses these effects, favoring the reorganization of periodontal ligament. Clinical relevance: Our results suggest that the application of orthodontic force in diabetic patients would increase inflammation and delay periodontal restructuring. Insulin would partly reverse this situation although glycemic decompensation episodes may occur. For these reasons, the periods between fixed orthodontic appliance activations should be of sufficient duration to allow adequate tissue recovery. | es |
| dc.format | application/pdf | es |
| dc.format.extent | 12 | es |
| dc.identifier.citation | Clinical Oral Investigations 2021 25: 1383-1394 | |
| dc.identifier.doi | https://doi.org/10.1007/s00784-020-03446-7 | |
| dc.identifier.issn | 436-3771 | |
| dc.identifier.issn | 1432-6981 | |
| dc.identifier.uri | http://hdl.handle.net/10201/137806 | |
| dc.language | eng | es |
| dc.relation | Sin financiación externa a la Universidad | es |
| dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | |
| dc.title | Effects of diabetes on oxidative stress, periodontal ligament fiber orientation and matrix metalloproteinase 8 and 9 expression during orthodontic tooth movement | es |
| dc.type | info:eu-repo/semantics/article | es |
| dspace.entity.type | Publication | es |
| relation.isAuthorOfPublication | 41991b8b-3a95-46de-ab2a-b7968ee121e6 | |
| relation.isAuthorOfPublication | c084ce85-206c-418d-8cec-7f85a0f34dc0 | |
| relation.isAuthorOfPublication.latestForDiscovery | 41991b8b-3a95-46de-ab2a-b7968ee121e6 |
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