Publication: A sex-related difference in the hypertrophic versus hyperplastic response of vascular smooth muscle cells to repeated passaging in culture
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Date
2001
Authors
Bkákovál, L. ; Pellicciari, C. ; Bottone, M.G. ; Lisá, V. ; Mares, V.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Activation of growth of vascular smooth
muscle cells (VSMC) in adults participates in
pathogenesis of dysplastic diseases of the vascular
system. In this study, we examined the impact of gender
of rat donors on the degree of hyperplastic and
hypertrophic responses of VSMC in cultures subjected
to repeated passaging. The cells were derived from the
outgrowth zone of explants of the thoracic aorta and
were studied up to passage 45. Under these conditions,
the cells undergo repeated growth stimulation by the
serum growth factors mimicking some pathological
situations in vivo. At lower passages (5-7), the cells
from both sex donors did not differ significantly in their
doubling time, maximum population density, protein
content and ploidy. At higher passages (40-45), we
found that the hyperplastic response, monitored by
doubling time and BrdU-revealed DNA synthesis, was
more intense in VSMC of male origin. In contrast,
female-derived cells reacted by more prominent
hypertrophic changes. The latter included a relatively
higher increase in the volume and protein content of
cells. As indicated by the DNA content histograms and
chromosome numbers, these cells also showed a higher
degree of passage-dependent polyploidization. In
addition, the female-derived VSMC were found to be
more effective in adhesion to the growth support
evidenced by wider spreading and higher resistance of
these cells to trypsin-mediated detachment as well as
higher expression of some integrin and cytoskeletal
molecules. These features could partly account for the
slower proliferation and polyploidization of these cells.
The results suggest that rat VSMC populations of male
and female origin contain cells which are intrinsically
different with respect to their capability of reacting to
growth stimuli. The lower responsiveness of femalederived
cells to growth stimuli may contribute to less
frequent formation of hyperplastic vascular lesions in female organisms.
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