Publication: A change in the localization of the region trapping
immune complexes in rat popliteal lymph nodes
during development of germinal centers, with regard
to the distribution of follicular dendritic cells
Authors
Sato, H. ; Dobashi, Michio ; Maeda, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A study was conducted to clarify changes in
the relationship between the region of irnrnune complex
(IC) trapping by follicular dendritic cells (FDCs) and the
distribution of FDC during reaction of germinal centers
(GCs), and to examine the relationship between the
tridiniensional shape of the IC-trapping regions and their
two-dimensional shape. Five-week-old rats were given
footpad injections of sheep red blood cells, and then
their popliteal lymph nodes were excised between days O
and 42, 24 h after injection of peroxidase-antiperoxidase
complex (PAP) as an IC. The specimens were
immunostained for PAP trapping on serial paraffin
sections, and for S-100 protein as a marker of FDCs. It
was found that during the GC reaction, PAP trapping
became weak and then disappeared on the basal side of
developing GCs where S-100 protein-positive FDCs
were still present. Al1 of the 1933 lymph follicles
examined were found to trap PAP. Whereas the
tridiniensional shapes of the trapping regions showed
similar pattems according to the development of lymph
follicles, their two-dimensional shapes varied. We
suggest that FDCs in primary follicles may differentiate
into FDCs in the light zone and FDCs in the dark zone in
secondary follicles. To evaluate each of the
compartments of a lymph follicle more accurately,
investigators should pay attention to the tridimensional
shape of the compartment.
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